FRAP analysis of the stability of the microtubule population along the neurites of chick sensory neurons.
FRAP analysis of the stability of the microtubule population along the neurites of chick sensory neurons.
复制标题
沿鸡感觉神经元神经突微管群稳定性的 FRAP 分析。
DOI：
10.1002/cm.970250108
复制
发表时间：
1993
影响因子：
--
通讯作者：
Letourneau,PC
中科院分区：
文献类型：
--
作者：
Edson,KJ;Lim,SS;Borisy,GG;Letourneau,PC
为了研究微管在伸长的神经突中的周转，用x-罗丹明微管蛋白显微注射鸡胚感觉神经元，6-12小时后，沿沿着选择的神经突的短节段在多个位点被光漂白。先前的研究[Lim等人，1989; 1990]表明，在神经突中荧光的恢复（FRAP）通过静止微管的动态更新而发生。在所有情况下，远端漂白区恢复荧光快于漂白区更接近位于沿着相同的神经突。生长锥的漂白区在30-40分钟内完全恢复，而位于更近端的漂白区通常在50-120分钟内恢复。在长神经突的最近端区域，荧光的恢复通常是不完全的，这表明在这些区域中的微管的显著部分是非常稳定的。这些研究表明，微管的稳定性沿着生长神经突的长度存在差异。这些差异可能是由以下因素的综合作用引起的：（1）在成熟神经突中稳定和延长微管的修饰，以及（2）远端定向微管加末端的动态不稳定性。
In order to study microtubule turnover in elongating neurites, chick embryo sensory neurons were microinjected with x-rhodamine tubulin, and after 6–12 hours, short segments along chosen neurites were photobleached at multiple sites. Previous studies [Lim et al., 1989; 1990] indicated that recovery of fluorescence (FRAP) in neurites occurs by the dynamic turnover of stationary microtubules. In all cases, distal bleached zones recovered fluorescence faster than bleached zones more proximally located along the same neurites. Bleached zones at growth cones completely recovered in 30–40 minutes, while bleached zones located more proximally usually recovered in 50–120 minutes. In the most proximal regions of long neurites, recovery of fluorescence was often incomplete, indicating that a significant fraction of the microtubules in these regions were very stable. These studies indicate that there are differences in microtubule stability along the lenght of growing neurites. These differences may arise from the combined effects of (1) modifications that stabilize and lengthen microtubules in maturing neurites and (2) the dynamic instability of the distally oriented microtubule plus ends.© 1993 Wiley-Liss, Inc.