Characterization of Organotin-Resistant Bacteria from Boston Harbor Sediments
Characterization of Organotin-Resistant Bacteria from Boston Harbor Sediments
复制标题
波士顿港沉积物中有机锡抗性细菌的表征
DOI：
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发表时间：
1998
影响因子：
4
通讯作者：
J. Cooney
中科院分区：
文献类型：
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作者：
A. Pain;J. Cooney
摘要：有机锡广泛应用于农业和工业。它们对包括细菌在内的各种生物体都有毒性，尽管对其生理学和生态学知之甚少。从美国马萨诸塞州波士顿港沉积物中分离出对三丁基锡（TBT）、二丁基锡（DBT）、一丁基锡（MBT）、三苯基锡（TPT）、二苯基锡（DPT）和一苯基锡（MPT）6种有机锡具有耐药性的细菌。从河口沉积物中直接分离出对除DPT外的每种有机锡具有抗性的细菌。在实验室中连续转移的有机锡抗性细菌的存活率为80%至91%。每种分离物都被筛选出对其他有机锡的抗性。所有250株分离株均对至少两种有机锡耐药。虽然其他有机锡耐药菌中存在DPT耐药，但在DPT初始分离时未发现DPT耐药菌株。80%的三丁基锡化合物耐药性细菌对三丁基锡化合物也有耐药性，这表明含有三丁基锡化合物的油漆在用于抑制微生物生物膜的油漆中并不是三丁基锡化合物的合适替代品。在某些情况下，脱丁基降低了毒性，而脱苯基则没有。因此，尽管三取代的有机锡通常被认为比二取代或单取代的有机锡更具毒性，但情况可能并非总是如此，并且可能涉及一种以上的耐药性机制。所有的细菌都对测试的8种重金属中的至少6种有抗性，这表明对重金属的抗性可能与对有机锡的抗性有关。
Abstract.Organotins are widely used in agriculture and industry. They are toxic to a variety of organisms including bacteria, although little is known of their physiology and ecology. Bacteria resistant to six organotins—tributyltin (TBT), dibutyltin (DBT), monobutyltin (MBT), triphenyltin (TPT), diphenyltin (DPT), and monophenyltin (MPT)—were isolated from Boston Harbor sediments, Massachusetts, USA. Bacteria resistant to each of the organotins, except DPT, were isolated directly from estuarine sediments. Viability of the organotin-resistant bacteria on serial transfer in the laboratory ranged from 80 to 91%. Each isolate was screened for resistance to the other organotins. All of 250 isolates were resistant to at least two organotins. No DPT-resistant isolates were found on initial isolation on DPT, although there was DPT resistance among the other organotin-resistant bacteria. Eighty percent of TBT-resistant bacteria were TPT-resistant, suggesting that antifouling paints containing TPT will not be a suitable substitute for TBT in paints designed to inhibit microbial biofilms. Debutylation reduced toxicity in some cases while dephenylation did not. Thus, even though trisubstituted organotins are generally believed to be more toxic than di- or monosubstituted organotins, this may not always be the case, and more than one mechanism of resistance may be involved. All the bacteria were resistant to at least six of eight heavy metals tested, suggesting that resistance to heavy metals may be associated with resistance to organotins.