TRACHEAL EPITHELIAL DAMAGE ALTERS TRACER FLUXES AND EFFECTS OF TRACHEAL OSMOLALITY IN SHEEP IN-VIVO
TRACHEAL EPITHELIAL DAMAGE ALTERS TRACER FLUXES AND EFFECTS OF TRACHEAL OSMOLALITY IN SHEEP IN-VIVO
复制标题
DOI：
10.1152/jappl.1995.78.5.1921
复制
发表时间：
1995-05-01
影响因子：
3.3
通讯作者：
WIDDICOMBE, JG
中科院分区：
文献类型：
--
作者：
WELLS, UM;WOODS, AJ;WIDDICOMBE, JG
Tracheal osmolality affects blood flow and the flux of a tracer, technetium-99m-labeled diethylenetriamine pentaacetic acid (Tc-99m-DTPA), from tracheal lumen to venous blood in anesthetized sheep. Hyperosmolar liquids increase blood flow and slightly decrease Tc-99m-DTPA flux, whereas hyposmolar liquids have no effect on blood flow and greatly increase Tc-99m-DTPA flux. We have now investigated whether epithelial damage induced by exposure of the tracheal lumen to a detergent (0.2% Triton X-100) alters these effects. A tracheal artery was perfused, and tracheal venous blood was collected. The initial tracheal volume was 12.8 +/- 0.7 ml. Triton X-100 greatly increased the permeability coefficient for Tc-99m-DTPA from -2.1 X 10(-7) to -240 x 10(-7) cm/s. Hyperosmolar Krebs-Henseleit solution (KH; 739 +/- 6 mosmol/kg) increased arterial (+14.3%) and venous (+21.5%) flows and decreased Tc-99m-DTPA output by 51.7%. Water flux into the lumen (+0.3 +/- 0.1 ml) was not significant, and the osmolality decreased by 99 +/- 9 mosmol/kg. Hyposmolar KH (124 +/- 2 mosmol/kg) had no effect on arterial and venous flows (-1.3% for both), and the increase in Tc-99m-DTPA output (+8.3%) was small and not significant. The volume decreased by 0.4 +/- 0.1 ml, and the osmolality increased by 36 +/- 4 mosmol/kg. Thus epithelial damage greatly increases the baseline permeability of the tracheal wall to Tc-99m-DTPA. It does not alter the qualitative effects of hyperosmolar KH on blood flow and Tc-99m-DTPA output but does reduce the effect of hyposmolar KH on Tc-99m-DTPA output. The latter effect may be a consequence of the reduced net water movement in response to non-isosmolar solutions after epithelial damage.
