Comparison of nasopharyngeal nylon flocked swabs with universal transport medium and rayon-bud swabs with a sponge reservoir of viral transport medium in the diagnosis of paediatric influenza
Comparison of nasopharyngeal nylon flocked swabs with universal transport medium and rayon-bud swabs with a sponge reservoir of viral transport medium in the diagnosis of paediatric influenza
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DOI：
10.1099/jmm.0.015305-0
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发表时间：
2010-01-01
影响因子：
3
通讯作者：
Principi, Nicola
中科院分区：
文献类型：
--
作者：
Esposito, Susanna;Molteni, Claudio Giuseppe;Principi, Nicola
本研究比较了用于儿童流感病毒分子检测的试剂盒（包含鼻咽尼龙植绒拭子和装有液体通用转运培养基（UTM）的试管）与试剂盒（包含带有海绵病毒转运培养基储存器的塑料轴人造丝出芽拭子）。使用两种拭子以随机顺序采集314名5岁以下流感样疾病儿童（186名男性;平均年龄2.32 ± 2.27岁）的呼吸道样本。植绒拭子可检出28例甲型流感（8.9%）和45例B型流感（14.3%）病例，人造丝拭子可检出26例甲型流感（8.3%）和43例B型流感（13.7%）病例，检出率分别为23.2%和22.0%，循环阈值相似。儿科医生和实验室工作人员对UTM鼻咽植绒拭子的简单性（P < 0.0001）和快速性（P < 0.0001）的满意度明显更高。这些发现表明，带有UTM的植绒拭子和带有海绵转运培养基的人造纤维芽拭子在保存流感病毒核酸方面具有相似的效率，但是含有带有UTM的植绒拭子的试剂盒允许更容易和更快速地收集和处理标本。
This study compared a kit containing a nasopharyngeal nylon flocked swab and a tube with a liquid universal transport medium (UTM) with a kit containing a plastic-shafted rayon-budded swab with a sponge reservoir of viral transport medium for the molecular detection of influenza viruses in children. Respiratory samples were collected from 314 children aged < 5 years with influenza-like illness (186 males; mean age 2.32 +/- 2.27 years) using both swabs in a randomized sequence for each patient. The flocked swabs permitted the detection of 28 influenza A (8.9%) and 45 influenza B (14.3%) cases, and the rayon-bud swabs 26 influenza A (8.3%) and 43 influenza B (13.7%) cases, with detection rates of 23.2 and 22.0%, respectively, and similar cycle threshold values. Paediatricians and laboratory staff were significantly more satisfied with both the simplicity (P < 0.0001) and rapidity (P < 0.0001) of the nasopharyngeal flocked swabs with UTM. These findings show that the flocked swabs with UTM and the rayon-bud swabs with a sponge transport medium are similarly efficient in preserving influenza virus nucleic acid, but that the kit containing a flocked swab with a UTM allows easier and more rapid collection and processing of specimens.