TEMPERATURE-DERIVATIVE SPECTROSCOPY - A TOOL FOR PROTEIN DYNAMICS
TEMPERATURE-DERIVATIVE SPECTROSCOPY - A TOOL FOR PROTEIN DYNAMICS
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DOI：
10.1073/pnas.87.1.1
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发表时间：
1990-01-01
影响因子：
11.1
通讯作者：
BRAUNSTEIN, D
中科院分区：
文献类型：
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作者：
BERENDZEN, J;BRAUNSTEIN, D
引入了一种测量群体相对于温度的导数的松弛方法，并用于研究光解闪光后二氧化碳与抹香鲸肌红蛋白的重组。对红外 CO 伸缩带中的成对过程的测量显示了每个带具有不同分布的分布式活化焓、对应于光解配体的两个带之间的转变以及动力学烧孔。高斯熵分布很好地描述了数据；结果与等温方法的结果相匹配并补充。温度导数技术进一步用于探索血红素袋外 CO 的重组。
A relaxation method that measures the derivative of a population with respect to temperature is introduced and used to study the recombination of CO to sperm whale myoglobin after a photolyzing flash. Measurement of the geminate process in the infrared CO-stretch bands shows distributed activation enthalpies with different distributions for each band, transitions between two bands that correspond to photolyzed ligands, and kinetic hole burning. The data are well described by gaussian enthalpy distributions; the results match and complement those of isothermal methods. The temperature-derivative technique is further used to explore the recombination of CO from outside the heme pocket.