Separation of large quantities of chromosomes by velocity sedimentation at unit gravity.
Separation of large quantities of chromosomes by velocity sedimentation at unit gravity.
复制标题
通过单位重力下的速度沉降分离大量染色体。
DOI：
10.1016/0014-4827(80)90485-1
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发表时间：
1980
影响因子：
3.7
通讯作者：
J. Boezeman
中科院分区：
文献类型：
--
作者：
John G. Collard;A. Tulp;J. H. Hollander;F. W. Bauer;J. Boezeman
介绍了一种基于单位重力速度沉降的染色体分选技术，该技术既简单又分辨率高。大量的染色体（多达1010条）可以被分类用于进一步的生物和生化研究。雄性印度麂细胞首先同步，随后在长春花碱有丝分裂中积累。低渗处理后，有丝分裂细胞被破坏，在一个特别设计的沉降室（面积127.6 cm2）中，粗染色体制备物作为薄膜分层在两步线性蔗糖梯度上。在单位重力下沉淀18 h后，收集各组分，用流式细胞术和显微镜分析各组分的染色体组成。
A chromosome sorting technique is described based on velocity sedimentation at unit gravity which combines simplicity with good resolution. Large quantities of chromosomes (up to 1010) can be sorted for further biological and biochemical research. Male Indian muntjac cells were first synchronized and subsequently accumulated in mitosis with vinblastine. After hypotonic treatment, mitotic cells were disrupted and the crude chromosomal preparation was layered as a thin film onto a two-step linear sucrose gradient in a specially designed sedimentation chamber (area 127.6 cm2). After 18 h of sedimentation at unit gravity, fractions were collected and the chromosomal composition of the fractions was analyzed by flow cytometry and microscopy.