A MICROSCOPE DIFFUSION CHAMBER FOR THE DETERMINATION OF THE EQUILIBRIUM AND NON-EQUILIBRIUM OSMOTIC RESPONSE OF INDIVIDUAL CELLS
A MICROSCOPE DIFFUSION CHAMBER FOR THE DETERMINATION OF THE EQUILIBRIUM AND NON-EQUILIBRIUM OSMOTIC RESPONSE OF INDIVIDUAL CELLS
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DOI：
10.1111/j.1365-2818.1985.tb02641.x
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发表时间：
1985-01-01
影响因子：
2
通讯作者：
MCGRATH, JJ
中科院分区：
文献类型：
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作者：
MCGRATH, JJ
已经开发出显微镜扩散室，可以直接观察微体积悬浮液中单个细胞的动态渗透响应。可以对静止细胞进行连续观察，包括在细胞外化学成分发生变化时缩短实验时间。溶质类型或浓度的多次变化很容易对单个样品体积施加。响应时间是腔室配置的函数，但在样品区域内溶质浓度梯度可以忽略不计的情况下，响应时间可以低至 1-10 秒。该室的构造和使用简单且经济。它的大小与普通玻璃载玻片相同，可以轻松适应任何常见的实验室显微镜。所有标准光学技术均可与腔室一起使用。讨论了施工细节和操作特征，包括重要的限制。包括显微照片和渗透数据的示例。
A microscope diffusion chamber has been developed which allows direct observation of the dynamic osmotic response of individual cells in micro-volume suspensions. Continuous observation of stationary cells is possible including short experimental times while the extracellular chemical composition is changed. Multiple changes of solute type or concentration are easily imposed upon a single sample volume. Response times are a function of chamber configuration but response times as low as 1-10 s are possible with negligible solute concentration gradients within the sample region. The chamber is simple and economical to construct and use. It is the size of an ordinary glass microslide and it can be adapted easily to any common laboratory microscope. All standard optical techniques may be used with the chamber. Construction details and operating characteristics including important limitations are discussed. Example photomicrographs and osmotic data are included.