Determination of the respiratory quotient in marine animals
Determination of the respiratory quotient in marine animals
复制标题
海洋动物呼吸商的测定
DOI：
10.1002/jcp.1030090107
复制
发表时间：
1936
期刊：
影响因子：
--
通讯作者：
W. R. Amberson
中科院分区：
文献类型：
--
作者：
M. Bosworth;H. O'Brien;W. R. Amberson
几乎没有关于海洋动物呼吸商的可靠测量记录。所使用的方法是费力的，并且结果是可变的和不确定的（参见Gardner等人，'22，' 23，参考旧文献）。只有在能够用瓦尔堡压力计或类似仪器进行研究的最小生物体的情况下，我们才有任何可靠的信息。尽管对比较生理学有兴趣，但对较大水生生物的呼吸作用还没有进行充分的研究，因此，我们试图将货车斯莱克测压血气仪（货车斯莱克和尼尔，'24;货车斯莱克，' 27）用于此类工作。我们已经取得了一定程度的成功，我们相信，值得该领域的其他工作人员考虑这种技术。自1929年夏天以来，我们一直在不时地试验这种方法。一些早期的测定是由海洋生物实验室生理学课程的学生做出的。查尔斯·赖特博士和阿尔伯特·赫格瑙尔博士在这些早期的测定中特别有帮助。Keys（'30）和Olehman（' 34）一直主张使用货车-Slyke装置进行这种研究，但他们都没有给出任何动物实验。然而，Ostanbul已经确定了分析氧的方法的有效性，
Almost no dependable measurements of the respiratory quotient in marine animals are on record. The methods used have been laborious and the results variable and uncertain (see Gardner et al.,'22,'23, for references to the older literature). Only in the case of the very smallest organisms, capable of investigation by means of the Warburg manometer, or similar apparatus, do we have any reliable information. The respiration of the larger aquatic forms has not been adequately studied, in spite of its interest to comparative physiology.We have therefore attempted to adapt the Van Slyke manometric blood gas apparatus (Van Slyke and Neill,'24; Van Slyke,'27) to use in such work. We have secured a degree of success which, we believe, warrants the consideration of this technique by other workers in the field. We have been testing the method from time to time since the summer of 1929. Some of the earlier determinations were made by students in the physiology course at the Marine Biological Laboratory. Dr. Charles Wright and Dr. Albert Hegnauer were particularly helpful in these earlier determinations. The use of the Van Slyke apparatus for such work has been urged by Keys ('30) and by Oesting ('34), but neither gives any animal experiments. Oesting has, however, established the validity of the method for the analysis of oxygen and