The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana.
The Subcellular Localization and Functional Analysis of Fibrillarin2, a Nucleolar Protein in Nicotiana benthamiana.
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本塞姆氏烟草核仁蛋白 Fibrillarin2 的亚细胞定位及功能分析
DOI：
10.1155/2016/2831287
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发表时间：
2016
影响因子：
--
通讯作者：
Xie L
中科院分区：
文献类型：
--
作者：
Zheng L;Yao J;Gao F;Chen L;Zhang C;Lian L;Xie L;Wu Z;Xie L
核仁蛋白在植物细胞学、生长发育中起着重要作用。Fibrillarin 2是本氏烟草（Nicotianabenthamiana）的一种核仁蛋白。benthamiana）。用RT-PCR方法扩增其cDNA，并将其插入到黄色荧光蛋白（YFP）标记的表达载体pEarley 101中。融合蛋白定位于烟草叶表皮细胞的核仁和Cajal小体中。本萨米亚纳了N.本塞姆氏原纤维蛋白2（NbFib 2）蛋白具有三个功能结构域（即，富含甘氨酸和精氨酸的结构域、RNA结合结构域和α-螺旋结构域）和C-末端的核定位信号（NLS）。蛋白质三维结构分析预测NbFib 2为α/β蛋白。此外，使用病毒诱导的基因沉默（VIGS）方法来确定NbFib 2的功能。结果表明，NbFib 2基因沉默的N.本萨米亚纳
Nucleolar proteins play important roles in plant cytology, growth, and development. Fibrillarin2 is a nucleolar protein of Nicotiana benthamiana (N. benthamiana). Its cDNA was amplified by RT-PCR and inserted into expression vector pEarley101 labeled with yellow fluorescent protein (YFP). The fusion protein was localized in the nucleolus and Cajal body of leaf epidermal cells of N. benthamiana. The N. benthamiana fibrillarin2 (NbFib2) protein has three functional domains (i.e., glycine and arginine rich domain, RNA-binding domain, and α-helical domain) and a nuclear localization signal (NLS) in C-terminal. The protein 3D structure analysis predicted that NbFib2 is an α/β protein. In addition, the virus induced gene silencing (VIGS) approach was used to determine the function of NbFib2. Our results showed that symptoms including growth retardation, organ deformation, chlorosis, and necrosis appeared in NbFib2-silenced N. benthamiana.