Water extract of Cordyceps sinensis (WECS) inhibits the RANKL-induced osteoclast differentiation
Water extract of Cordyceps sinensis (WECS) inhibits the RANKL-induced osteoclast differentiation
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DOI：
10.1002/biof.5520300204
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发表时间：
2007-01-01
期刊：
影响因子：
6
通讯作者：
Takeda, Eiji
中科院分区：
文献类型：
--
作者：
Mizuha, Yoko;Yamamoto, Hironori;Takeda, Eiji
据报道，冬虫夏草是一种传统中药，具有多种药理作用。本研究旨在探讨冬虫夏草水提物（WECS）对体外培养的破骨细胞分化的影响。在小鼠骨髓细胞和单核细胞/巨噬细胞系RAW 264. 7中，WECS剂量依赖性地抑制核因子κ B（NF-κ B）配体（RANKL）受体激活剂诱导的破骨细胞分化（通过抗酒石酸酸性磷酸酶（TRAP）染色）。事实上，在用WECS处理的RAW 264.7细胞中未观察到细胞毒性效应。破骨细胞相关基因（降钙素受体、组织蛋白酶K、基质金属蛋白酶9和活化T细胞核因子c1）的mRNA表达也受到WECS的抑制。电泳迁移率变动分析（EMSA）和Western blot分析表明，WECS通过抑制I κ B α的磷酸化而抑制NF-κ B的活化。总之，本研究结果首次证明WECS是RANKL诱导的破骨细胞分化的有效抑制剂，其机制涉及NF-κ B通路。
It has been reported that Cordyceps sinensis, a traditional Chinese medicine, has various pharmacological effects. The aim of this study was to clarify the effect of water extract of Cordyceps sinensis (WECS) on osteoclast differentiation in vitro. In mouse bone marrow cells and monocyte/macrophage cell line RAW264.7, WECS dose-dependently inhibited the receptor activator of nuclear factor kappaB(NF-kappa B) ligand (RANKL)-induced osteoclast differentiation by tartrate-resistant acid phosphatase (TRAP) staining. In fact, cytotoxic effect was not observed in the RAW264.7 cells treated with WECS. Moreover, the mRNA expression of osteoclast related genes (calcitonin receptor, cathepsin K, matrix metalloprotease 9 and nuclear factor of activated T cells c1) was also inhibited by WECS. Investigation of inhibitory mechanism by using electrophoretic mobility shift assay (EMSA) and Western blot analysis revealed that WECS inhibited the activation of NF-kappa B through the prevention of I kappa B alpha phosphorylation. In conclusion, the present results demonstrate for the first time that WECS is a potent inhibitor of the RANKL-induced osteoclast differentiation through a mechanism involving the NF-kappa B pathway.