Histone Deubiquitinase OTU1 Epigenetically Regulates DA1 and DA2, Which Control Arabidopsis Seed and Organ Size
Histone Deubiquitinase OTU1 Epigenetically Regulates DA1 and DA2, Which Control Arabidopsis Seed and Organ Size
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DOI：
10.1016/j.isci.2020.100948
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发表时间：
2020-03-27
期刊：
影响因子：
5.8
通讯作者：
Citovsky, Vitaly
中科院分区：
文献类型：
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作者：
Keren, Ido;Lacroix, Benoit;Citovsky, Vitaly
种子是植物生命周期和人类营养的核心，是人类能量摄入的主要来源。为了更好地理解表观遗传标记的酶写者和抹除者的作用，特别是组蛋白泛素化和相应的组蛋白修饰剂，参与控制种子发育，我们鉴定了OTU1样半胱氨酸蛋白酶OTU1是一种组蛋白去泛素化酶，参与调节拟南芥种子和器官大小的DA1和DA2基因的转录抑制。OTU1的功能缺失突变体在DA1和DA2染色质中积累了H2B单泛素化和H3三甲基化和高乙酰化等共色标记。这些数据通过认识到OTU1是一个推定的抑制因子复合体的成员，负调控DA1和DA2基因的转录，提高了我们对DA1和DA2基因表观遗传调控的认识。
Seeds are central to plant life cycle and to human nutrition, functioning as the major supplier of human population energy intake. To understand better the roles of enzymic writers and erasers of the epigenetic marks, in particular, histone ubiquitylation and the corresponding histone modifiers, involved in control of seed development, we identified the otubain-like cysteine protease OTU1 as a histone deubiquitinase involved in transcriptional repression of the DA1 and DA2 genes known to regulate seed and organ size in Arabidopsis. Loss-of-function mutants of OTU1 accumulate H2B monoubiquitylation and such euchromatic marks as H3 trimethylation and hyperacetylation in the DA1 and DA2 chromatin. These data advance our knowledge about epigenetic regulation of the DA1 and DA2 genes by recognizing OTU1 as a member of a putative repressor complex that negatively regulates their transcription.