Genomic assignment of the warfarin resistance locus, Rw, in the rat
Genomic assignment of the warfarin resistance locus, Rw, in the rat
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DOI：
10.1007/s003359901073
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发表时间：
1999-07-01
期刊：
影响因子：
2.5
通讯作者：
Pelz, JH
中科院分区：
文献类型：
--
作者：
Kohn, MH;Pelz, JH
将抗凝血剂华法林和溴敌隆抗性位点（位点符号Rw）整合到大鼠（Rartus norvegicus）微卫星基因组图谱中，对两种化合物均具有抗性的分离品系大鼠的第7代后代进行了凝血反应（BCR）试验。未观察到对华法林和溴敌隆的抗性之间的重组，表明共同的遗传基础。在位于MIT微卫星图谱位置95.90（SHRSP x BN F-2-杂交）或82.24（FHH x ACI F-2-杂交）的Rw和D1 Arb 18（Myl 2）之间未发现重组体。抗性分离的比例预期为单一的，显性基因的反应。相同数量的女性和男性是耐药的，但女性抗凝剂给药后保留较高的百分比凝血活性（PCA）。大鼠、小鼠和人之间的部分同线性表明Myl 2可以用作定位小鼠和人中的Rw同源物的锚。
The locus responsible for resistance to the anticoagulants warfarin and bromadiolone (locus symbol Rw) was integrated into the rat (Rarttus norvegicus) microsatellite genome map, Seventh-generation offspring of a segregating strain of rats heterozygous resistant to both compounds were tested with a blood-clotting-response (BCR) test. No recombination between resistance to warfarin and bromadiolone was observed, indicating a common genetic basis. No recombinants were found between Rw and D1Arb18 (Myl2) located at the MIT-microsatellite map position 95.90 (SHRSP x BN F-2-cross) or 82.24 (FHH x ACI F-2-cross). Resistance segregated in a ratio expected for single, dominant gene responses. An equal number of females and males were resistant, but females retained higher percentage blood coagulation activities (PCA) after anticoagulant administration. Partial synteny between rat, mouse, and human suggests that Myl2 may serve as anchor to map the Rw homologs in mouse and human.