Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte
Think Negative: Finding the Best Electrospray Ionization/MS Mode for Your Analyte
复制标题
DOI：
10.1021/acs.analchem.7b00096
复制
发表时间：
2017-06-06
影响因子：
7.4
通讯作者：
Kruve, Anneli
中科院分区：
文献类型：
--
作者：
Liigand, Piia;Kaupmees, Karl;Kruve, Anneli
这是第一次将正负模式下的电喷雾电离效率（IE）刻度统一到一个系统中，从而能够直接比较不同电离模式下的IE值。这是通过使用在正和负模式下电离程度相似的参比化合物而实现的。因此，选择最佳（即，最灵敏的）电离条件。在两种模式下电离的33种化合物的电离效率表明，与一般实践相反，负模式允许46%的此类化合物具有更好的灵敏度，而正模式仅优选18%，并且对于36%，两种模式的结果是相当的。
For the first time, the electrospray ionization efficiency (IE) scales in positive and negative mode are united into a single system enabling,direct comparison of IE values across ionization modes. This is made possible by the use of a reference Compound that ionizes to a similar extent in both positive, and negative modes. Thus, choosing the optimal (i.e., most sensitive) ionization conditions for a given set of analytes is enabled. Ionizatiori efficiencies of 33 compounds ionizing in both modes demonstrate that, contrary to general practice, negative mode allows, better sensitivity for 46% of such compounds Whereas the positive mode is preferred for only 18%, and for 36%, the results for both modes are comparable.