Brain recruitment of dendritic cells following Li-pilocarpine induced status epilepticus in adult rats
Brain recruitment of dendritic cells following Li-pilocarpine induced status epilepticus in adult rats
复制标题
锂-毛果芸香碱诱导成年大鼠癫痫持续状态后脑部树突状细胞的募集
DOI：
10.1016/j.brainresbull.2012.11.007
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发表时间：
2013-02-01
影响因子：
3.8
通讯作者：
Jiang, Wen
中科院分区：
文献类型：
--
作者：
Li, Xiao-Wei;Yang, Feng;Jiang, Wen
炎症和免疫途径的激活可能有助于癫痫的发展。树突状细胞（Dendritic cells，DCs）是一组异质性的专职抗原提呈细胞，在多种炎症状态下可在脑内检测到。然而，糖尿病诱导的DCs在脑内聚集的时空分布和起源尚未被探索。在本研究中，我们证明了CD 11 c阳性树突状细胞在海马，丘脑和颞叶皮质的存在下，Li-pilocarpine诱导癫痫持续状态（SE）大鼠。DC的募集早在诱导SE后1天发生，在第3天达到峰值时间，并且直到第5天仍然明显。氯化锂给药后未检测到脑中DC的募集。所观察到的DC不能用Iba-1（一种活化的小胶质细胞标记物）双标记，并且全身辐射防止了脑实质中脑损伤诱导的DC积累。我们的数据表明，DC在癫痫脑中的募集可能来自外周循环，这群免疫细胞可能参与SE后的免疫过程。(C)2012 Elsevier Inc. All rights reserved.
Activation of inflammatory and immune pathways may contribute to the development of epilepsy. Dendritic cells (DCs), a heterogeneous group of professional antigen presenting cells, can be detected in the brain under several inflammatory conditions. However, the spatiotemporal distribution and origin of seizure-induced DCs accumulation in the brain have not been explored yet. In the present study, we demonstrate the presence of CD11c-positive DCs in the hippocampus, thalamus and temporal cortex following Li-pilocarpine induced status epilepticus (SE) in rats. Recruitment of DCs occurs as early as 1 day following the induction of SE, reaching peak time at day 3, and still evident until day 5. The recruitment of DCs in the brain following lithium chloride administration was not detected. The observed DCs cannot be double-labeled with Iba-1 (an activated microglia marker) and whole-body radiation prevents seizure-induced DCs accumulation in brain parenchyma. Our data suggest that the recruitment of DCs in the epileptic brain may be derived from peripheral circulation and this population of immune cells may be involved in the immune processes after SE. (C) 2012 Elsevier Inc. All rights reserved.