Diverse patterns of expression of the cytochrome c oxidase subunit I gene and unassigned reading frames 4 and 5 during the life cycle of Trypanosoma brucei.
Diverse patterns of expression of the cytochrome c oxidase subunit I gene and unassigned reading frames 4 and 5 during the life cycle of Trypanosoma brucei.
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布氏锥虫生命周期中细胞色素 c 氧化酶亚基 I 基因和未指定阅读框 4 和 5 的不同表达模式。
DOI：
10.1128/mcb.5.11.3041-3047.1985
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发表时间：
1985
影响因子：
5.3
通讯作者：
Stuart,K
中科院分区：
文献类型：
--
作者：
Jasmer,DP;Feagin,JE;Stuart,K
研究了布氏锥虫164的一个大环片段的转录，该片段含有与细胞色素氧化酶亚基I（COI）和其他线粒体系统的未分配阅读框架（URFs）4和5相对应的核苷酸（nt）序列。两个主要的转录本，大小不同的ca。200 nt映射到COI和URF 4基因中的每一个，而单个主要转录本映射到URF 5。在总RNA中，较大的COI转录物在前循环形式（PF）中比在血流形式（BF）中更丰富，较小的COI和两个URF 4转录物在两种形式中具有相似的丰度，并且单个URF 5转录物在BF中比PF更丰富。这些表达模式在多聚腺苷酸+RNA中不同，这是由于多聚腺苷酸+线粒体转录物在PF中的比例比在BF中更高。此外，小（300- 500-nt）的RNA，从位于推定的蛋白质编码基因之间的富含C的序列转录也表现出不同的生活周期阶段之间的表达模式和差异的多聚腺苷酸化的PFs相比，BF。这些观察结果表明，多个过程调节线粒体基因的差异表达在布氏锥虫。
Transcription of a maxicircle segment fromTrypanosoma brucei164 that contains nucleotide (nt) sequences corresponding to cytochromecoxidase subunit I (COI) and unassigned reading frames (URFs) 4 and 5 of other mitochondrial systems was investigated. Two major transcripts that differ in size by ca. 200 nt map to each of the COI and URF4 genes, while a single major transcript maps to URF5. In total RNA, the larger COI transcript is more abundant in procyclic forms (PFs) than in bloodstream forms (BFs), the smaller COI and both URF4 transcripts have similar abundances in both forms, and the single URF5 transcript is more abundant in BF than PF. These patterns of expression differ in poly(A)+RNA as a result of a higher proportion of poly(A)+mitochondrial transcripts in PFs than in BFs. In addition, small (300- to 500-nt) RNAs that are transcribed from C-rich sequences located between putative protein-coding genes also exhibit diverse patterns of expression between life cycle stages and differences in polyadenylation in PFs compared with BFs. These observations suggest that multiple processes regulate the differential expression of mitochondrial genes inT. brucei.