Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities
Lactobacillus spp. bacteria as probiotics in gilthead sea bream (Sparus aurata, L.) larvae: Effects on growth performance and digestive enzyme activities
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DOI：
10.1016/j.aquaculture.2008.04.020
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发表时间：
2008-08
期刊：
影响因子：
4.5
通讯作者：
Cüneyt Suzer;Deniz Çoban;H. Kamacı;Şahin Saka;Kürşat Fırat;Özge Otgucuoğlu;Hakan Küçüksari
中科院分区：
文献类型：
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作者：
Cüneyt Suzer;Deniz Çoban;H. Kamacı;Şahin Saka;Kürşat Fırat;Özge Otgucuoğlu;Hakan Küçüksari
研究了添加商品益生菌乳杆菌对金枪鱼幼体发育过程中生长参数和消化酶活性的影响。所有试验设计为三个不同剂量的益生菌，从孵化后3天开始(DAH)，同时开始外源饲养。在第一组中，将益生菌添加到活食物(轮虫和卤虫)中。在第二组中，将益生菌直接添加到活的食物和水中。在第三组中，直接向水中添加益生菌。对照组不给予益生菌治疗。添加益生素组仔鱼水体和消化道的细菌总数与对照组差异显著(p<；0.05)。在海水中，对照组细菌总数的平均值约为试验组的4×104倍(p<；0.05)。此外，各益生菌处理组的平均消化酶活性与对照组差异显著(p<；0.05)。除补加益生素水组外，各处理组仔鱼的胰酶和肠酶比活力均显著高于添加活饲料和加水活饲料的仔鱼(p<；0.05)。此外，用含水的活体食物喂食益生菌的金黄色葡萄球菌幼虫，其存活率(比对照高13-105%)和比生长率(比对照高2-9%)都有显著提高(p<；0.05)。因此，在池塘直投水中添加益生菌并不能显著提高仔猪的生长参数和消化酶活性，因此，在养殖参数和营养条件方面，添加益生菌并不是有效的。
In this study, the influence of commercial probiotic, Lactobacillus spp., supplementation was investigated on growth parameters and digestive enzyme activities in gilthead sea bream, Sparus aurata, during larval development. All experiments were triplicated and designed in three different administrations of probiotic from 3 days after hatching (DAH) concurrently with starting of exogenous feeding. In the first group, probiotic was added to live food (rotifer and Artemia). In the second group, probiotic was supplemented directly to both live food and water. In the third group, probiotic was added directly to water. Also, no probiotic treatment was maintained in control group. Total bacterial counts among probiotic probiotic-supplemented groups were significantly different from total bacterial counts in controls in water and digestive tract of larvae (p<0.05). The mean of total bacterial counts in control was approximately 4×104-fold increased from the experimental groups in the sea water (p<0.05). Besides, mean digestive enzyme activities of all probiotics treatment groups were significantly different (p<0.05) with that of the control. Except probiotic water supplementation group, in all treatments, the specific activities of pancreatic and intestinal enzymes were significantly higher (p<0.05) in larvae to which probiotic had been supplemented by live food and live food with water. Also, S. aurata larvae that had probiotic administered by live food with water demonstrated significant (p<0.05) increases in both survival (13–105% higher) and specific growth rate (2–9% higher) as compared to controls. As a result, supplementation of probiotic to directly tank water could not significantly increase growth parameters and digestive enzyme activities and therefore, administration of probiotics by this method would not be effective in terms of husbandry parameters and nutritional condition.