Postovulatory aging causes the deterioration of porcine oocytes via induction of oxidative stress
Postovulatory aging causes the deterioration of porcine oocytes via induction of oxidative stress
复制标题
排卵后衰老通过诱导氧化应激导致猪卵母细胞恶化。
DOI：
10.1096/fj.201700908r
复制
发表时间：
2018-03-01
期刊：
影响因子：
4.8
通讯作者：
Xiong, Bo
中科院分区：
文献类型：
--
作者：
Miao, Yilong;Zhou, Changyin;Xiong, Bo
据报道，在啮齿类动物中，排卵后衰老对受精能力和随后的胚胎发育有负面影响；然而，在此过程中分子和细胞的变化尚未完全确定。在这里，我们使用猪卵母细胞（一种生理和发育与人类相似的模型）来探索排卵后衰老如何影响卵母细胞质量和受精能力的分子机制。我们发现，排卵后衰老导致猪卵母细胞的形态变化，表现为卵丘细胞的不致密扩张和破碎的增加。衰老还会破坏细胞器结构，包括纺锤体组装、肌动蛋白聚合和线粒体完整性，从而损害卵母细胞的质量。此外，排卵后衰老导致皮质颗粒和卵泡他星分布异常，进而导致精子结合缺陷，从而降低受精潜力。值得注意的是，我们观察到排卵后衰老诱导了氧化应激，高水平的活性氧和卵母细胞凋亡率，从而导致维持卵母细胞质量和受精能力的关键因素恶化。综上所述，我们的研究结果表明，排卵后衰老通过诱导氧化应激扰乱了猪卵母细胞的各种分子和细胞变化。-苗，杨，周，春，崔，忠，张，明，石阳，旭，陆勇，熊斌。排卵后衰老通过诱导氧化应激导致猪卵母细胞退化。
Negative effects of postovulatory aging on fertilization ability and subsequent embryo development have been reported in rodents; however, the molecular and cellular changes during this process have not been fully defined. Here, we used porcine oocytes, a model that is physiologically and developmentally similar to humans, to explore the molecular mechanisms that underlie how postovulatory aging affects oocyte quality and fertilization capacity. We found that postovulatory aging caused the morphologic change of porcine oocytes by exhibiting the incompact expansion of cumulus cells and an increased occurrence of fragmentation. Aging also impaired oocyte quality by disrupting organelle structures, including the spindle assembly, actin polymerization, and mitochondrial integrity. Moreover, postovulatory aging led to the abnormal distribution of cortical granules and ovastacin, which, in turn, resulted in defective sperm binding and consequently compromised fertilization potential. Of note, we observed that postovulatory aging induced oxidative stress with a high level of reactive oxygen species and apoptotic rate in oocytes, thereby resulting in the deterioration of critical factors in the maintenance of oocyte quality and fertilization capacity. Taken together, our findings demonstrate that postovulatory aging perturbs a variety of molecular and cellular changes in porcine oocytes by inducing oxidative stress.-Miao, Y., Zhou, C., Cui, Z., Zhang, M., ShiYang, X., Lu, Y., Xiong, B. Postovulatory aging causes the deterioration of porcine oocytes via induction of oxidative stress.