Characteristics of hepatitis B virus genotype G coinfected with genotype H in chimeric mice carrying human hepatocytes
Characteristics of hepatitis B virus genotype G coinfected with genotype H in chimeric mice carrying human hepatocytes
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DOI：
10.1016/j.virol.2008.04.001
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发表时间：
2008-07-05
期刊：
影响因子：
3.7
通讯作者：
Mizokami, Masashi
中科院分区：
文献类型：
--
作者：
Tanaka, Yasuhito;Sanchez, Laura V.;Mizokami, Masashi
积累的证据表明，乙型肝炎病毒基因型 G (HBV/G) 仅存在于与其他 HBV 基因型的合并感染中。在墨西哥，6 名男男性行为者的 HBV/G 与 HBV/H 合并感染。 6 种墨西哥 HBV/G 毒株的系统发育完整基因组与之前来自美国/欧洲的毒株密切相关。使用具有人类肝细胞的uPA/SCID小鼠，HBV/G的单一感染不会导致血清中检测到HBV DNA，而在接种HBV/H的第10周时，当HBV/H DNA升高至>10(7)拷贝/mL时，HBV/G的双重感染增强了HBV/G的复制。另外 3 个接种含有 HBV/G 和微量 HBV/H 的血清传代的 HBV/G 得到增强。小鼠同时感染 HBV/G 和 H 会导致肝脏纤维化。综上所述，HBV/G与HBV/H共感染时，复制能力显着增强。在免疫抑制条件下，与 HBV/G 共感染可能直接导致细胞病变。 (C) 2008 Elsevier Inc. 保留所有权利。
Accumulated evidence indicated that hepatitis B virus genotype G (HBV/G) is present exclusively in coinfection with other HBV genotypes. In Mexico, HBV/G from 6 men who had sex with men were coinfiected with HBV/H. Phylogenetically complete genomes of the 6 Mexican HBV/G strains were closely related to previous ones from the US/Europe. Using uPA/SCID mice with human hepatocytes, monoinfection with HBV/G did not result in detectable HBV DNA in serum, whereas superinfection with HBV/G at week 10 inoculated HBV/H when HBV/H DNA was elevated to >10(7) copies/mL has enhanced the replication of HBV/G. The HBV/G was enhanced in another 3 inoculated with a serum passage containing HBV/G with a trace of HBV/H. Coinfection of mice with HBV/G and H induced fibrosis in the liver. In conclusion, the replication of HBV/G can be enhanced remarkably when it is coinfected with HBV/H. Coinfection with HBV/G may be directly cytopathic in immunosuppressive conditions. (C) 2008 Elsevier Inc. All rights reserved.