Translocatable leaf signal autoregulates soybean nodulation
Translocatable leaf signal autoregulates soybean nodulation
复制标题
可移位的叶片信号自动调节大豆结瘤
DOI：
10.1016/0168-9452(95)04107-6
复制
发表时间：
1995
期刊：
影响因子：
5.2
通讯作者：
J. E. Harper
中科院分区：
文献类型：
--
作者：
P. Francisco;J. E. Harper
大豆（Glycine max（L.）Merr.），过度增殖表型的表达似乎受芽控制。当前的研究试图定位大豆品种中结瘤信号的来源。Williams 82及其高增殖突变体NOD 1 -3。利用楔形嫁接（整个拍摄的替代品）和方法嫁接（除了拍摄）和拍摄（接穗）的治疗范围从完整的拍摄到各种组合的叶，子叶，和顶端从嫁接接穗去除。带叶楔形嫁接或近接接穗类型决定了寄主植物的分枝表型。具有更多叶子的接穗在改变再生方面显然更有效。接穗与叶和无尖是有效的，在改变开花接穗与叶和尖。这证实了Delves等人的报道（Plant Cell Environ.，15（1992）249-254），但在这两种情况下，结果都不是确定的，因为新芽继续形成，这可能为控制结瘤提供刺激。还检查了被刺激形成根的叶和枝插穗的结瘤表达。没有分生组织顶端的叶插表现出与嫩枝插相似的分化表型，即威廉姆斯82的叶插和嫩枝插正常分化，而NOD 1 -3的叶插和嫩枝插过度分化。切叶的方法明确表明，叶，而不是顶端，是一个可转位的信号控制结节数量的自动调节的合成网站。
In soybean (Glycine max (L.) Merr.), expression of the hypernodulation phenotype appears to be controlled by the shoot. The current study attempted to localize the source of the nodulation signal in soybean cv. Wiliams 82 and its hypernodulating mutant NOD1-3. Wedge grafts (replacement of entire shoot) and approach grafts (addition of a shoot) were utilized and the shoot (scion) treatments ranged from complete shoots to various combinations of leaf, cotyledon, and apex removal from the grafted scion. Leaf-bearing wedge-grafted or approach-grafted scion types dictated the nodulation phenotype of the host plant. Scions with more leaves were clearly more effective in altering nodulation. Scions with leaves and without apices were as effective in altering nodulation as were scions with both leaves and apices. This confirmed the report by Delves et al. (Plant Cell Environ., 15 (1992) 249–254), but in neither case are results definitive as new buds continued to form which may provide stimulus for nodulation control. Leaf and shoot cuttings which were stimulated to form roots were also examined for expression of nodulation. Leaf cuttings, which were devoid of any meristematic apices, exhibited nodulation phenotypes similar to that of the shoot cuttints, i.e. leaf and shoot cutting of Williams 82 were normally nodulated while those of NOD1-3 were hypernodulated. The leaf cutting approach definitively showed that the leaf, and not the apex, was the synthesis site of a translocatable signal controlling autoregulation of nodule number.