The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.
The Trichomonas vaginalis hydrogenosome proteome is highly reduced relative to mitochondria, yet complex compared with mitosomes.
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DOI：
10.1016/j.ijpara.2011.10.001
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发表时间：
2011-11
影响因子：
4
通讯作者：
Johnson PJ
中科院分区：
文献类型：
--
作者：
Schneider RE;Brown MT;Shiflett AM;Dyall SD;Hayes RD;Xie Y;Loo JA;Johnson PJ
人类病原体阴道毛滴虫缺乏常规的线粒体，而是含有不同的尿道相关细胞器。这些双膜结合的细胞器，称为氢小体，产生分子氢。氢化酶体的系统发育和生物化学分析表明，与线粒体的共同起源，但氢化酶体蛋白的鉴定及其代谢的研究一直是有限的。在这里，我们提供了一个详细的蛋白质组学分析的T。迷走氢小体纯化的氢化酶体的蛋白质组由569种蛋白质组成，这一数字大大低于真菌和动物线粒体蛋白质组报道的1，000 - 1，500种蛋白质，但大大高于有丝分裂体的蛋白质。氢酶体蛋白质组揭示了线粒体和有丝分裂体共同和不同的途径。已知在氨基酸和能量代谢、Fe-S簇组装、黄素介导的催化、氧应激反应、膜转位、伴侣蛋白功能、蛋白水解加工和ATP水解中起作用的蛋白质占氢酶体蛋白质组的约30%。在氢酶体蛋白质组中的569种蛋白质中，许多似乎与氢酶体的外表面相关，包括大量的GTP酶和核糖体蛋白。糖酵解蛋白也被发现与氢酶体蛋白质组，类似于以前观察到的线粒体蛋白质组。大约18%的氢酶体蛋白质组由未知功能的假设蛋白质组成，预测这些高度适应的细胞器的多种活性和性质尚未被发现。
The human pathogen Trichomonas vaginalis lacks conventional mitochondria and instead contains divergent mitochondrial-related organelles. These double-membrane bound organelles, called hydrogenosomes, produce molecular hydrogen. Phylogenetic and biochemical analyses of hydrogenosomes indicate a common origin with mitochondria; however identification of hydrogenosomal proteins and studies on its metabolism have been limited. Here we provide a detailed proteomic analysis of the T. vaginalis hydrogenosome. The proteome of purified hydrogenosomes consists of 569 proteins, a number substantially lower than the 1,000 - 1,500 proteins reported for fungal and animal mitochondrial proteomes, yet considerably higher than proteins assigned to mitosomes. Pathways common to and distinct from both mitochondria and mitosomes were revealed by the hydrogenosome proteome. Proteins known to function in amino acid and energy metabolism, Fe-S cluster assembly, flavin-mediated catalysis, oxygen stress response, membrane translocation, chaperonin functions, proteolytic processing and ATP hydrolysis account for ∼30% of the hydrogenosome proteome. Of the 569 proteins in the hydrogenosome proteome, many appear to be associated with the external surface of hydrogenosomes, including large numbers of GTPases and ribosomal proteins. Glycolytic proteins were also found to be associated with the hydrogenosome proteome, similar to that previously observed for mitochondrial proteomes. Approximately 18% of the hydrogenosomal proteome is composed of hypothetical proteins of unknown function, predictive of multiple activities and properties yet to be uncovered for these highly adapted organelles.
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影响因子：
64.8
作者：
Boxma, B;de Graaf, RM;Hackstein, JHP
通讯作者：
Hackstein, JHP
影响因子：
4.8
作者：
Coombs, GH;Westrop, GD;Müller, S
通讯作者：
Müller, S
影响因子：
64.8
作者：
Dyall, SD;Yan, WH;Johnson, PJ
通讯作者：
Johnson, PJ
影响因子：
9.2
作者：
Chan, KW;Slotboom, DJ;Tovar, J
通讯作者：
Tovar, J
DOI：
10.1073/pnas.0500349102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Dolezal, P;Smid, O;Tachezy, J
通讯作者：
Tachezy, J