Xenohybrid Bone Graft Containing Intrinsically Disordered Proteins Shows Enhanced In Vitro Bone Formation
Xenohybrid Bone Graft Containing Intrinsically Disordered Proteins Shows Enhanced In Vitro Bone Formation
复制标题
含有本质无序蛋白质的异种混合骨移植物显示出增强的体外骨形成
DOI：
10.1021/acsabm.0c00064
复制
发表时间：
2020
影响因子：
4.7
通讯作者：
Håvard Jostein Haugen
中科院分区：
文献类型：
--
作者：
Hao Zhu;Manuel Gomez;Jun Xiao;Giuseppe Perale;Felice Betge;Ståle Petter Lyngstadaas;Håvard Jostein Haugen
骨缺损是世界范围内的一个重大健康问题，因为骨是仅次于血液的第二大移植组织。虽然无数的骨移植物（BG）已被用于治疗骨修复，但没有一种具有所有理想的特性。改善BG的一种方法是添加生物活性组分，然而通常是困难的，因为BG的产生可能破坏这些分子的生物活性。在这里，我们提出了一种复合异种杂交BG，SmartBonePep，具有一种受内在无序蛋白质（IDP）启发的生物分子。这些合成肽（命名为P2和P6）被物理地包埋到复合物BG的聚合物基质中。测试了SmartBonePep对人成骨细胞的影响。结果表明，SmartBonePep增强了增殖和成骨作用。为了验证P2和P6的生物活性，通过将这些肽也加入到细胞培养基中来间接测试。P2或P6对成骨相关基因表达有促进作用。在这项研究中，我们展示了高效的骨诱导合成肽P2或P6，它们具有富含脯氨酸和内在无序的结构特征。IDP的这种使用可以为临床使用提供有前景的骨增强生物分子。
Bone defects are a significant health problem worldwide, as bone is the second-most transplanted tissue after blood. Although a myriad of bone grafts (BGs) have been used to treat bone repairs, none of them possesses all the desirable characteristics. An approach to improve BGs is to add bio-active components, however often difficult as BG production may disrupt the biological activities of such molecules. Here, we present a composite xenohybrid BG, SmartBonePep, with a type of biomolecule inspired by intrinsically disordered proteins (IDPs). These synthetic peptides (named P2 and P6) are physically entrapped into the polymer matrix of the composite BG. The effects of SmartBonePep on human osteoblasts were tested. Results showed that SmartBonePep enhanced proliferation and osteogenic effects. In order to verify the bioactivity of P2 and P6, these peptides were tested indirectly by being added to cell culture media too. Here, P2 or P6 exhibited promoting effects on osteogenic-related gene expressions. In this study, we showed highly effective osteoinductive synthetic peptides P2 or P6, which possess proline-rich and intrinsically disordered structural characters. This use of IDPs may provide promising bone enhancement biomolecules for clinical usage.