Development and characterization of a silica monolith immobilized enzyme micro-bioreactor
Development and characterization of a silica monolith immobilized enzyme micro-bioreactor
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DOI：
10.1021/ie049354f
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发表时间：
2005-01-05
影响因子：
4.2
通讯作者：
Ijima, H
中科院分区：
文献类型：
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作者：
Kawakami, K;Sera, Y;Ijima, H
用正硅氧基硅烷和甲基三甲氧基硅烷1：4的溶胶-凝胶原位转化法制备了硅胶整体固定化酶，填充了内径为0.1~2.0 mm的10 cm长的聚醚醚酮(PEEK)毛细管。以20 mM(S)-(-)缩水甘油与0.4M丁酸乙烯酯在有机溶剂中的酯交换反应为考察对象。底物溶液以0.0004~5.0mLmin(-1)的流速流过色谱柱。在高液体流量下，微生物反应器的转化率高于间歇式反应器。当三个管子串联时，酶的质量与液体流量的固定比例下的转化率提高了约50%，这是因为与单管相比，流量增加了三倍。在表观液速一定的情况下，管径的变化对转化率没有影响。此外，转化率随着酶含量的降低而增加。这些结果归因于液固传质的明显效应，并用一个简单的数学模型进行了定量分析。
Several 10-cm-long capillary tubes [made of poly(ether ether ketone) (PEEK)] with inside diameters of 0.1-2.0 mm were filled with silica monolith-immobilized protease derived by in situ sol-gel transition from a 1:4 mixture of tetramethoxysilane and methyltrimethoxysilane. Transesterification between 20 mM (S)-(-)-glycidol and 0.4 M vinyl n-butyrate in an organic solvent was used as the test reaction. The substrate solution flowed through the column at a flow rate of 0.0004-5.0 mL(.)min(-1). The conversion in the micro-bioreactor was higher than that in the batch reactor at a high liquid flow rate. When three tubes were connected in series, the conversion at a fixed ratio of the mass of the enzyme to the liquid flow rate was increased by approximately 50%, because of the tripling of the flow rate as compared to the case with a single tube. Changes in the tube diameter had no influence on the conversion at a fixed superficial liquid velocity. Further, the conversion increased with a decrease in the enzyme content. These results were ascribed to the apparent effect of liquid-solid mass transfer and were analyzed quantitatively using a simple mathematical model.