Generation by reverse genetics of an effective attenuated Newcastle disease virus vaccine based on a prevalent highly virulent Chinese strain
Generation by reverse genetics of an effective attenuated Newcastle disease virus vaccine based on a prevalent highly virulent Chinese strain
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DOI：
10.1007/s10529-015-1799-z
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发表时间：
2015-06-01
影响因子：
2.7
通讯作者：
Zhang, Guo-Zhong
中科院分区：
文献类型：
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作者：
Liu, Meng-Meng;Cheng, Jin-Long;Zhang, Guo-Zhong
为探讨纽卡斯尔流行株与疫苗株之间的差异是否是导致鸡新城疫爆发的原因，本研究利用流行株VIId分离株SG 10构建了反向遗传学系统，并将其命名为aSG 10，通过将F蛋白切割位点的强毒基序“（112）RRQKRa匕首“F-117”改造为无毒基序“（112）GRQGR α匕首“L-117”，建立了一个新的F蛋白切割位点的强毒基序。从平均死亡时间和脑内致病指数证实了aSG 10的减毒致病性。aSG 10和LaSota均保护接种疫苗的禽类在用高毒力基因型VII NDV，SG 10株攻击后免于死亡。然而，与LaSota疫苗相比，aSG 10显著减少了从接种疫苗的禽类的攻击病毒脱落。我们还产生了一个重组病毒，aSG 10增强的绿色荧光蛋白（EGFP），表达EGFP。aSG 10-EGFP突变体可稳定表达EGFP，并可安全地作为疫苗载体，为提高疫苗的免疫保护力提供了一种新的疫苗候选基因。
To investigate whether the differences between the circulating Newcastle disease virus (NDV) isolates and the used vaccine might account for the current ND outbreaks in vaccinated poultry flocks.A reverse genetics system using prevalent genotype VIId isolate SG10 was constructed and a mutant virus, named aSG10, was developed by changing the virulent F protein cleavage site motif "(112)RRQKRa dagger"F-117" into an avirulent motif "(112)GRQGRa dagger"L-117". The attenuated pathogenicity of aSG10 was confirmed from the mean death time and intracerebral pathogenicity index. aSG10 and LaSota both protected vaccinated birds from death after challenge with highly virulent genotype VII NDV, strain SG10. However, aSG10 significantly reduced the challenge virus shedding from the vaccinated birds compared to LaSota vaccine. We also generated a recombinant virus, aSG10-enhanced green fluorescent protein (EGFP), which expresses EGFP. aSG10-EGFP stably expressed EGFP for at least 10 passages.The mutant, aSG10, can be safely used as a vaccine vector and is a potential vaccine candidate in increasing the protective efficacy for the control of current ND epidemic in China.