Establishment and characterization of human colorectal cancer cell lines.
Establishment and characterization of human colorectal cancer cell lines.
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DOI：
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发表时间：
1984-12
期刊：
影响因子：
11.2
通讯作者：
J. A. McBain;J. Weese;L. Meisner;W. Wolberg;J. Willson
中科院分区：
文献类型：
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作者：
J. A. McBain;J. Weese;L. Meisner;W. Wolberg;J. Willson
描述了从新鲜手术切除的组织中建立人结直肠癌细胞系的方案。从18种原发癌中的6种和4种转移癌中的4种建立了12种人结直肠癌细胞系。从两个个体患者建立来自并发原发性肿瘤和转移的细胞系。两种原发性癌症产生了具有不同生物学特性的多种细胞系。促成我们成功的因素似乎是基于底物粘附和通过时间的差异选择。该方案避免使用饲养层或通过无胸腺小鼠传代。所建立的细胞系表现出一系列核型、形态和生长特征。
A protocol for establishment of human colorectal carcinoma cell lines from fresh surgically removed tissues is described. Twelve human colorectal carcinoma cell lines were established from 6 of 18 primary cancers and four of four metastases. Cell lines from concurrent primary tumors and metastases were established from two individual patients. Two primary cancers gave rise to multiple cell lines with differing biological characteristics. Factors contributing to our success appear to be differential selection on the basis of substrate adherence and the timing of passage. The protocol avoids the use of feeder layers or passage through athymic mice. The established cell lines exhibit a range of karyotypes, morphologies, and growth characteristics.