Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry
Development and validation of a quantitative method for the determination of 12 endocannabinoids and related compounds in human plasma using liquid chromatography-tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2009.04.010
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发表时间：
2009-05-15
影响因子：
3
通讯作者：
Witkamp, Renger F.
中科院分区：
文献类型：
--
作者：
Balvers, Michiel G. J.;Verhoeckx, Kitty C. M.;Witkamp, Renger F.
开发并验证了用于定量人血浆中内源性大麻素和相关结构大麻素、2-花生四烯酰甘油、2-花生四烯酰甘油醚、O-花生四烯酰乙醇酰胺、二高-γ-亚麻酰乙醇酰胺、二十二碳四烯酰乙醇酰胺、N-花生四烯酰多巴胺、N-花生四烯酰甘氨酸、N-油酰多巴胺、油酰乙醇酰胺、棕榈酰乙醇酰胺和硬脂酰乙醇酰胺的灵敏度和特异性LC-MS/MS方法。使用乙腈提取化合物，然后进行固相萃取。在Xterra C8柱上使用梯度洗脱偶联三重四极杆MS进行分离。LLOQ水平范围为0.02至1.75 μ g/mL，LOD范围为0.0002至0.1266 ng/mL，所有加标水平下的准确度均>80%（最低加标水平下的硬脂酰乙醇酰胺除外）。(C)2009 Elsevier B. V.保留所有权利。
A sensitive and specific LC-MS/MS method for the quantification of the endocannabinoids and related structures anandamide, 2-arachidonoyl glycerol, 2-arachidonyl glycerol ether, O-arachidonoyl ethanolamide, dihomo-gamma-linolenoyl ethanolamide, docosatetraenoyl ethanolamide, N-arachidonoyl dopamine, N-arachidonyl glycine, N-oleoyl dopamine, oleoyl ethanolamide, palmitoyl ethanolamide, and stearoyl ethanolamide in human plasma was developed and validated. Compounds were extracted using acetonitrile followed by solid-phase extraction. Separation was performed on a Xterra C8 column using gradient elution coupled to a triple-quadrupole MS. LLOQ levels ranged from 0.02 to 1.75 mu g/mL, LODs ranged from 0.0002 to 0.1266 ng/mL, and accuracies were >80% (except stearoyl ethanolamide at lowest spike level) at all spike levels. (C) 2009 Elsevier B.V. All rights reserved.