Persistent changes in corticotropin-releasing factor neuronal systems induced by maternal deprivation.
Persistent changes in corticotropin-releasing factor neuronal systems induced by maternal deprivation.
复制标题
DOI：
10.1210/endo.137.4.8625891
复制
发表时间：
1996-04
期刊：
影响因子：
4.8
通讯作者：
Charlotte O. Ladd;M. Owens;C. Nemeroff
中科院分区：
文献类型：
--
作者：
Charlotte O. Ladd;M. Owens;C. Nemeroff
有相当多的证据表明，CRF神经元整合内分泌，自主神经，免疫和行为反应的压力。在这项研究中，我们研究了长期的影响，早期应激对发育中的下丘脑和下丘脑外CRF神经系统在大鼠大脑和随后的反应，在成年人的压力。具体而言，我们试图确定是否成年雄性大鼠先前隔离6小时，每天在出生后第2-20天的反应，在一个生物化学不同的方式，以一个温和的足部冲击应力相比，控制。四个治疗组进行了检查：非剥夺（NDEP）/无休克，NDEP/休克，剥夺（DEP）/无休克，和DEP/休克。与NDEP组相比，DEP大鼠的基础和应激诱导的ACTH浓度均升高。此外，DEP大鼠表现出增加125%的免疫反应CRF浓度的正中隆起和减少CRF受体结合的密度在垂体前叶相比，在所有NDEP大鼠。下丘脑外CRF系统的改变也很明显，在DEP与NDEP动物，观察到的CRF受体结合位点中缝核的数量增加了59%，臂旁核的免疫反应性CRF浓度增加了86%。这些结果表明，母鼠断奶前在雄性大鼠的CRF神经系统的中枢神经系统和垂体，是明显的几个月后，可能与持续的行为反应在成年大鼠的改变产生影响。
There is considerable evidence that CRF-containing neurons integrate the endocrine, autonomic, immune, and behavioral responses to stress. In this study we examined long term effects of early stress on developing hypothalamic and extrahypothalamic CRF neural systems in the rat brain and subsequent responses to stress in the adult. Specifically, we sought to determine whether adult male rats previously isolated for 6 h daily during postnatal days 2-20 react in a biochemically distinct manner to a mild foot shock stress compared to controls. Four treatment groups were examined: nondeprived (NDEP)/no shock, NDEP/shock, deprived (DEP)/no shock, and DEP/shock. Compared to the NDEP group, DEP rats exhibited an increase in both basal and stress-induced ACTH concentrations. Moreover, DEP rats exhibited a 125% increase in immunoreactive CRF concentrations in the median eminence and a reduction in the density of CRF receptor binding in the anterior pituitary compared to those in all NDEP rats. Alterations in extrahypothalamic CRF systems were also apparent in DEP vs. NDEP animals, with an observed 59% increase in the number of CRF receptor-binding sites in the raphe nucleus and an 86% increase in immunoreactive CRF concentrations in the parabrachial nucleus. These results indicate that maternal deprivation before weaning in male rats produces effects on CRF neural systems in both the central nervous system and pituitary that are apparent several months later and are probably associated with persistent alterations in behavioral response in adult rats.