EXPRESSION OF THE BETA-NERVE GROWTH-FACTOR GENE IN HIPPOCAMPAL-NEURONS
EXPRESSION OF THE BETA-NERVE GROWTH-FACTOR GENE IN HIPPOCAMPAL-NEURONS
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DOI：
10.1126/science.2897715
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发表时间：
1988-06-03
期刊：
影响因子：
56.9
通讯作者：
PERSSON, H
中科院分区：
文献类型：
--
作者：
AYERLELIEVRE, C;OLSON, L;PERSSON, H
使用神经生长因子 (NGF) 互补 DNA 探针进行原位杂交来鉴定大鼠和小鼠脑中含有 NGF 信使 RNA 的细胞。最强烈的标记发生在海马体中，在齿状回和锥体细胞层中发现了杂交神经元。通过海马区 NGF 杂交 mRNA 的损失进一步评估含有 NGF mRNA 的细胞的神经元身份，其中神经元已被红藻氨酸或秋水仙碱破坏。 RNA印迹分析还显示，在秋水仙碱产生损伤后，大鼠齿状回中NGF mRNA的水平显着降低。这种病变还导致 Thy-1 mRNA 水平下降和胶质纤维酸性蛋白 mRNA 水平增加。因此，神经元死亡与 NGF mRNA 的消失有关。这些结果表明 NGF 由大脑中的神经元合成，并暗示在海马体中，NGF 通过神经元与神经元的相互作用影响 NGF 敏感神经元。
In situ hybridization with complementary DNA probes for nerve growth factor (NGF) was used to identify cells containing NGF messenger RNA in rat and mouse brain. The most intense labeling occurred in hippocampus, where hybridizing neurons were found in the dentate gyrus and the pyramidal cell layer. The neuronal identity of NGF mRNA-containing cells was further assessed by a loss of NGF-hybridizing mRNA in hippocampal areas where neurons had been destroyed by kainic acid or colchicine. RNA blot analysis also revealed a considerable decrease in the level of NGF mRNA in rat dentate gyrus after a lesion was produced by colchicine. This lesion also caused a decrease in the level of Thy-1 mRNA and an increase in the level of glial fibrillary acidic protein mRNA. Neuronal death was thus associated with the disappearance of NGF mRNA. These results suggest a synthesis of NGF by neurons in the brain and imply that, in hippocampus, NGF influences NGF-sensitive neurons through neuron-to-neuron interactions.