Rodent epidermal Langerhans cells demonstrate greater histochemical specificity for ADP than for ATP and AMP.
Rodent epidermal Langerhans cells demonstrate greater histochemical specificity for ADP than for ATP and AMP.
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啮齿动物表皮朗格汉斯细胞对 ADP 的组织化学特异性高于对 ATP 和 AMP 的组织化学特异性。
DOI：
10.1111/1523-1747.ep12261031
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Bergstresser,PR
中科院分区：
文献类型：
--
作者：
Chaker,MB;Tharp,MD;Bergstresser,PR
哺乳动物表皮的朗格汉斯细胞（LCs）具有Ca++/Mg++依赖性腺苷三磷酸酶（ATPase），该酶在各种组织制剂中作为树突状细胞的有用组织化学标记物。由于atp酶仅代表几种潜在的细胞表面多磷酸酶之一，我们研究了3种相关腺嘌呤核苷酸底物识别啮齿动物表皮LCs的能力。细胞表面atp酶活性在瓦巴因的存在下不受抑制，并且观察到严格的二价阳离子依赖性，在ca++和mg++之间具有完全的互换性。两种阳离子的最佳染色浓度为20mm。底物浓度依赖性也被观察到，在0.33 mM腺苷5 ' -三磷酸（ATP）下染色效果最佳。然而，在等摩尔的基础上，5'-二磷酸腺苷（ADP）在鉴定整个表皮和分离的表皮细胞悬浮液中的lccs方面优于ATP。底物腺苷5′-单磷酸腺苷（AMP）在两种啮齿类动物中均能染色滤泡上皮细胞，但无法识别小鼠表皮的LCs，仅能微弱染色大鼠表皮的树突状细胞。我们从这些研究中得出结论，与ATP相比，ADP对LC表面多磷酸酶活性表现出更大的特异性，并且在表皮细胞悬液的鉴定过程中无意中包含AMP会错误地鉴定出LC以外的细胞。
Langerhans cells (LCs) in mammalian epidermis possess the ectoenzyme Ca++/Mg++-dependent adenosine triphosphatase (ATPase), which has served as a useful histochemical marker for these dendritic cells in a variety of tissue preparations. Since ATPase represents only one of several potential cell surface polyphosphatases, we investigated the capacities of 3 related adenine nucleotide substrates to identify rodent epidermal LCs. Cell surface ATPase activity was not inhibited in the presence of ouabain and was observed to be strictly divalent cation-dependent, with complete interchangeability between Ca++and Mg++. Optimal staining in the presence of either cation occurred at a 20 mM concentration. Substrate concentration dependence was also observed, with optimal staining at 0.33 mM adenosine 5′-triphosphate (ATP). On an equimolar basis, however, adenosine 5'-diphosphate (ADP) was superior to ATP for the identification of LCs both in whole mounts of epidermis and in suspensions of disaggregated epidermal cells. The substrate adenosine 5'-monophosphate (AMP) stained follicular epithelial cells in both rodent species but failed to identify epidermal LCs in the mouse and only weakly stained these dendritic cells in rat epidermis. We conclude from these studies that ADP demonstrates greater specificity for LC surface polyphosphatase activity than ATP and that the inadvertent inclusion of AMP during identification procedures for epidermal cell suspensions will falsely identify cells other than LCs.