Molecular histopathology by spectrally reconstructed nonlinear interferometric vibrational imaging.
Molecular histopathology by spectrally reconstructed nonlinear interferometric vibrational imaging.
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DOI：
10.1158/0008-5472.can-10-1554
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发表时间：
2010-12-01
期刊：
影响因子：
11.2
通讯作者：
Boppart SA
中科院分区：
文献类型：
--
作者：
Chowdary PD;Jiang Z;Chaney EJ;Benalcazar WA;Marks DL;Gruebele M;Boppart SA
用于快速定量分析组织切片、切除的组织标本或原位组织的灵敏测定对于早期疾病诊断是高度期望的。染色的组织病理学是金标准，但仍然是一个主观的做法，处理组织需要数小时至数天。我们描述了一种显微镜技术，获得一个敏感和准确的颜色编码的图像从内在的分子标记。光谱重建的非线性干涉振动成像可以在临床前大鼠乳腺癌模型中以大于99%的置信区间区分癌症与正常组织切片，并使用在不到5分钟内成像的新鲜未染色组织切片以大于99%的置信区间将癌症边界定义为±100 μm。通过优化光源和光束传输，该技术可以实现实时即时光学分子成像和诊断。
Sensitive assays for rapid quantitative analysis of histologic sections, resected tissue specimens, or in situ tissue are highly desired for early disease diagnosis. Stained histopathology is the gold standard but remains a subjective practice on processed tissue taking from hours to days. We describe a microscopy technique that obtains a sensitive and accurate color-coded image from intrinsic molecular markers. Spectrally reconstructed nonlinear interferometric vibrational imaging can differentiate cancer versus normal tissue sections with greater than 99% confidence interval in a preclinical rat breast cancer model and define cancer boundaries to ±100 μm with greater than 99% confidence interval, using fresh unstained tissue sections imaged in less than 5 minutes. By optimizing optical sources and beam delivery, this technique can potentially enable real-time point-of-care optical molecular imaging and diagnosis.