A common step for changing cell shape in fruiting body and starvation-independent sporulation of Myxococcus xanthus
A common step for changing cell shape in fruiting body and starvation-independent sporulation of Myxococcus xanthus
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DOI：
10.1128/jb.182.12.3553-3558.2000
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发表时间：
2000-06-01
影响因子：
3.2
通讯作者：
Kaiser, D
中科院分区：
文献类型：
--
作者：
Licking, E;Gorski, L;Kaiser, D
黄色粘球菌可以两种方式中的任一种形成孢子：在子实体发育程序结束时或在生长细胞暴露于某些试剂（如浓缩甘油）后。子实体孢子形成需要饥饿，而甘油孢子形成需要快速生长，并且由于两种类型的孢子在结构上有些不同，因此通常认为这两种过程是不同的。然而，已分离出Tn 5 Lac插入突变Omega 7536，其同时阻断子实体孢子以及甘油诱导的孢子的发育。在将杆状细胞转化为球形孢子的过程中，两种孢子形成途径在突变体中被阻断。Omega 7536基因座在适合于每个过程的细胞形状变化时表达，甘油诱导后早期和饥饿诱导后晚期。在C-信号反应途径中，可以确定Omega 7536基因座正常功能的位置和甘油诱导刺激的位置，这些位置是独特的，并且与观察结果一致。虽然这两种孢子形成途径在某些方面有所不同，但它们至少有一个共同的步骤，将杆状细胞转变为球形孢子。
Myxococcus xanthus can sporulate in either of two ways: at the end of the program of fruiting body development or after exposure of growing cells to certain reagents such as concentrated glycerol. Fruiting body sporulation requires starvation, while glycerol sporulation requires rapid growth, and since the two types of spores are structurally somewhat different, it has generally been assumed that the two processes are different. However, a Tn5 Lac insertion mutation, Omega 7536, has been isolated which simultaneously blocks the development of fruiting body spores as well as glycerol-induced spores, Both sporulation pathways are blocked in the mutant within the process that converts a rod-shaped cell into a spherical spore. The Omega 7536 Locus is expressed at the time of cell shape change appropriate to each process, early after glycerol induction and late after starvation induction. On the C-signal response pathway, it is possible to identify positions for the normal function of the Omega 7536 locus and for the inducing stimulus from glycerol that are unique and consistent with the observations. Although the two sporulation pathways differ in certain respects, it is shown that they share at least one step for changing a rod-shaped cell into a spherical spore.