A compact Acousto-Optic Lens for 2D and 3D femtosecond based 2-photon microscopy.
A compact Acousto-Optic Lens for 2D and 3D femtosecond based 2-photon microscopy.
复制标题
DOI：
10.1364/oe.18.013720
复制
发表时间：
2010-06-21
期刊：
影响因子：
3.8
通讯作者：
Silver RA
中科院分区：
文献类型：
--
作者：
Kirkby PA;Srinivas Nadella KM;Silver RA
介绍了一种用于飞秒双光子成像的高速三维声光透镜显微镜（AOLM）。通过优化4 AO偏转器（AODs）的设计，并通过推导新的控制算法，我们已经开发出一种紧凑的球形AOL，具有低的时间色散，使2光子成像的功率比以前报道的低10倍。我们表明，AOLM可以执行高速2D光栅扫描成像（>150 Hz），而没有扫描速率相关的散光。它可以在30 kHz的3D随机存取序列中偏转和聚焦激光束，并具有扩展的聚焦范围（>137 μm; 40 X 0.8NA物镜）。这些特征可能使AOLM成为研究分布在3D空间中的快速生理过程的有用工具
We describe a high speed 3D Acousto-Optic Lens Microscope (AOLM) for femtosecond 2-photon imaging. By optimizing the design of the 4 AO Deflectors (AODs) and by deriving new control algorithms, we have developed a compact spherical AOL with a low temporal dispersion that enables 2-photon imaging at 10-fold lower power than previously reported. We show that the AOLM can perform high speed 2D raster-scan imaging (>150 Hz) without scan rate dependent astigmatism. It can deflect and focus a laser beam in a 3D random access sequence at 30 kHz and has an extended focusing range (>137 μm; 40X 0.8NA objective). These features are likely to make the AOLM a useful tool for studying fast physiological processes distributed in 3D space