Distinct molecular features of colorectal carcinoma with signet ring cell component and colorectal carcinoma with mucinous component
Distinct molecular features of colorectal carcinoma with signet ring cell component and colorectal carcinoma with mucinous component
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DOI：
10.1038/modpathol.3800482
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发表时间：
2006-01-01
期刊：
影响因子：
7.5
通讯作者：
Fuchs, CS
中科院分区：
文献类型：
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作者：
Ogino, S;Brahmandam, M;Fuchs, CS
印戒细胞癌和粘液癌是结直肠腺癌的不同亚型。与非粘液腺癌相比，具有各种印戒细胞成分的结直肠癌和具有各种粘液成分的结直肠癌的形态学和分子光谱尚未被研究。研究组包括39个具有各种印戒细胞成分的癌（印戒组），167个具有各种粘液成分的癌（粘液组）和457个非粘液腺癌。我们目测估计肿瘤中印戒细胞和粘液成分的量，并根据每种成分的量（= 50%）对印戒细胞和粘液组进行细分。我们对BRAF和KRAS进行了测序，分析了微卫星不稳定性（MSI）和18 q杂合性缺失（洛），并对TP 53、环氧合酶-2（COX 2）、MLH 1、O-6-甲基鸟嘌呤DNA甲基转移酶（MGMT）、p16（CDKN 2A）和脂肪酸合成酶（FXR）进行了免疫组化。印戒细胞癌（>= 50%印戒细胞肿瘤）和= 50%粘液性肿瘤，
Signet ring cell carcinoma and mucinous carcinoma are distinct subtypes of colorectal adenocarcinoma. The morphologic and molecular spectra of colorectal carcinomas with various signet ring cell components and colorectal carcinomas with various mucinous components, compared to non-mucinous adenocarcinomas, have not been examined. The study groups consisted of 39 carcinomas with various signet ring cell components ('the signet group'), 167 carcinomas with various mucinous components ('the mucinous group'), and 457 nonmucinous adenocarcinoma. We visually estimated the amounts of signet ring cell and mucinous components in tumors, and subclassified the signet and mucinous groups according to the amount of each component (= 50%). We sequenced BRAF and KRAS, analyzed for microsatellite instability (MSI) and 18q loss of heterozygosity (LOH), and performed immunohistochemistry for TP53, cyclooxygenase-2 (COX2), MLH1, O-6-methylguanine DNA methyltransferase (MGMT), p16 (CDKN2A), and fatty acid synthase (FASN). Signet ring cell carcinoma (>= 50% signet ring cell tumors) and = 50% mucinous tumors and