Chromatin remodelling at promoters suppresses antisense transcription
Chromatin remodelling at promoters suppresses antisense transcription
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DOI：
10.1038/nature06391
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发表时间：
2007-12-13
期刊：
影响因子：
64.8
通讯作者：
Tsukiyama, Toshio
中科院分区：
文献类型：
--
作者：
Whitehouse, Iestyn;Rando, Oliver J.;Tsukiyama, Toshio
染色质允许真核细胞有效地包装其DNA。为了了解染色质结构是如何在酿酒酵母基因组中控制的，我们研究了ATP依赖性染色质重塑复合物Isw 2在定位核小体中的作用。我们发现，Isw2的功能启动子区域附近，它重新定位在基因和基因间序列之间的接口核小体。Isw 2的核小体重新定位是定向的，并导致基因间区域的核小体占据增加。Isw2活性的丧失导致不适当的转录，导致编码和非编码转录本的产生。在这里，我们表明，Isw2重新定位核小体，以加强方向性转录，防止转录起始的隐蔽网站。我们的分析揭示了染色质是如何在全球范围内组织起来的，并推进了我们对转录是如何调节的理解。
Chromatin allows the eukaryotic cell to package its DNA efficiently. To understand how chromatin structure is controlled across the Saccharomyces cerevisiae genome, we have investigated the role of the ATP- dependent chromatin remodelling complex Isw2 in positioning nucleosomes. We find that Isw2 functions adjacent to promoter regions where it repositions nucleosomes at the interface between genic and intergenic sequences. Nucleosome repositioning by Isw2 is directional and results in increased nucleosome occupancy of the intergenic region. Loss of Isw2 activity leads to inappropriate transcription, resulting in the generation of both coding and noncoding transcripts. Here we show that Isw2 repositions nucleosomes to enforce directionality on transcription by preventing transcription initiation from cryptic sites. Our analyses reveal how chromatin is organized on a global scale and advance our understanding of how transcription is regulated.