Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.
Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F(2alpha) in human urine using liquid chromatography-tandem mass spectrometry.
复制标题
使用液相色谱-串联质谱法对人尿液中的氧化损伤生物标志物 2,3-dinor-8-isoprostaglandin-F(2alpha) 进行定量。
DOI：
10.1016/j.ab.2009.12.024
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发表时间：
2010
影响因子：
2.9
通讯作者：
Millington,DavidS
中科院分区：
文献类型：
--
作者：
Zhang,Haoyue;Il'yasova,Dora;Sztaray,Judit;Young,SarahP;Wang,Frances;Millington,DavidS
F2-异前列腺素是人类氧化状态的有用生物标志物。我们开发了一种超高效液相色谱-串联质谱 (UPLC-MS/MS) 方法来定量 2,3-二去甲-8-异前列腺素 F2α（8-异前列腺素 F2α 的尿液代谢物）。通过固相萃取纯化尿液，并通过负离子电喷雾电离的 UPLC-MS/MS 进行分析。该方法稳健，平均误差为 9%，日间和日内误差为 7.5% 或更低，定量下限为 0.5 μg/L，相当于注入柱中的 0.04 pmol。 6 分钟的分析时间比之前发布的方法更短，并且适合大型研究。
F2-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography–tandem mass spectrometric (UPLC–MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F2α, a urinary metabolite of 8-iso-prostaglandin F2α.Urine was purified by solid-phase extraction and analyzed by UPLC–MS/MS with negative-ion electrospray ionization. The method was robust with a mean inaccuracy of 9%, interday and intraday imprecision of 7.5% or lower, and a lower limit of quantification of 0.5 μg/L, equivalent to 0.04 pmol injected onto the column. An analysis time of 6 min was shorter than previously published methods and amenable to large studies.