Potential role of the heat shock protein 90 (hsp90) in buffering mutations to favour cyclical parthenogenesis in the peach potato aphid Myzus persicae (Aphididae, Hemiptera)
Potential role of the heat shock protein 90 (hsp90) in buffering mutations to favour cyclical parthenogenesis in the peach potato aphid Myzus persicae (Aphididae, Hemiptera)
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DOI：
10.1017/s0007485318000688
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发表时间：
2019-08-01
影响因子：
1.9
通讯作者：
Manicardi, G. C.
中科院分区：
文献类型：
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作者：
Mandrioli, M.;Zanetti, E.;Manicardi, G. C.
热休克蛋白90 （hsp90）是一类能够稳定参与几个过程的“客户”蛋白网络的分子。此外，最近的研究表明，hsp90编码基因的突变诱导了广泛的表型异常，这被解释为不同发育途径对隐藏/隐藏突变的敏感性增加。为了验证hsp90在蚜虫中的作用，我们对桃薯蚜虫Myzus persicae （Sulzer, 1776）的17个世系进行了hsp90基因扩增和测序，寻找突变的存在。特别地，我们比较了具有不同生殖模式（专性与周期性孤雌生殖）的谱系，发展有翼雌性的倾向和核型稳定性。与具有功能性hsp90基因的周期性孤雌谱系不同，分析的7个无性谱系显示出严重的突变（包括移码和无义突变）。用hsp90抑制剂格尔达霉素进行的体内功能分析显示，在缺乏活性hsp90的情况下，一些具有周期性孤雌生殖的谱系可能会失去诱导性生殖的能力，这表明它们的基因组中存在隐性突变。总的来说，我们的数据表明hsp90可能在蚜虫中发挥缓冲隐藏/隐藏突变的作用，这些突变破坏了周期性孤雌生殖。
Heat-shock proteins 90 (hsp90s) are a class of molecules able to stabilize a network of 'client' proteins that are involved in several processes. Furthermore, recent studies indicated that mutations in the hsp90-encoding gene induce a wide range of phenotypic abnormalities, which have been interpreted as an increased sensitivity of different developmental pathways to hidden/cryptic mutations. In order to verify the role of hsp90 in aphids, we amplified and sequenced the hsp90 gene in 17 lineages of the peach potato aphid Myzus persicae (Sulzer, 1776) looking for the presence of mutations. In particular, we compared lineages with different reproductive modes (obligate vs. cyclical parthenogenesis), propensity to develop winged females and karyotype stability. Differently from the cyclical parthenogenetic lineages that possessed functional hsp90 genes, the seven analysed asexual lineages showed severe mutations (including frameshift and non-sense mutations). In vivo functional assays with the hsp90-inhibitor geldanamycin showed that some lineages with cyclical parthenogenesis may lose their ability to induce sexuales in the absence of active hsp90 revealing the presence of cryptic mutations in their genomes. As a whole, our data suggest that hsp90 could play in aphids a role in buffering hidden/cryptic mutations that disrupt cyclical parthenogenesis.