Cyclosporine a inhibits the mRNA expressions of IL-2, IL-4 and IFN-γ, but not TNF-α, in canine mononuclear cells
Cyclosporine a inhibits the mRNA expressions of IL-2, IL-4 and IFN-γ, but not TNF-α, in canine mononuclear cells
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DOI：
10.1292/jvms.69.887
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发表时间：
2007-09-01
影响因子：
1.2
通讯作者：
Iwasaki, Toshiroh
中科院分区：
文献类型：
--
作者：
Kobayashi, Tetsuro;Momoi, Yasuyuki;Iwasaki, Toshiroh
观察钙调神经磷酸酶抑制剂环孢素A(CsA)和FK506对犬多种细胞因子基因表达的影响，以确定CsA和FK506对犬的作用是否与人类相似。实时荧光定量RT-PCR检测CsA或FK506与PHA刺激的犬PBMC孵育5h后细胞因子IL-2、IL-4、干扰素-γ和肿瘤坏死因子-α的表达水平。两种试剂均以剂量依赖方式抑制IL-2、IL-4和1FN-Gamma的mRNA表达。而CsA对肿瘤坏死因子-α的表达无明显抑制作用。这些发现对于评估CsA治疗犬的适应症很重要。
The effects of the calcineurin inhibitors cyclosporine A (CsA) and FK506 on the mRNA expressions of various cytokines were evaluated in dogs to determine whether the effects of CsA and FK506 in dogs were similar to those in humans. The mRNA expression levels of the cytokines IL-2, IL-4, IFN-gamma and TNF-alpha were measured in PHA-stimulated canine PBMC using real-time RT-PCR after incubation with CsA or FK506 for 5 hr. Both reagents inhibited IL-2, IL-4 and 1FN-gamma mRNA expressions in a dose-dependent manner. However, CsA hardly inhibited the mRNA expression of TNF-alpha. These findings are important for assessing the indications of CsA treatment in dogs.