Surface modification of electrospun nanofibrous scaffolds via polysaccharide-protein assembly multilayer for neurite outgrowth
Surface modification of electrospun nanofibrous scaffolds via polysaccharide-protein assembly multilayer for neurite outgrowth
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DOI：
10.1039/c2jm32332j
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Zhou, Libing
中科院分区：
文献类型：
--
作者：
He, Liumin;Shi, Yunfeng;Zhou, Libing
在这项研究中，我们提出了一个简单而有效的方法来修改聚酯静电纺丝纤维支架多糖蛋白质多层形成的基础上静电相互作用。分别以壳聚糖（CS）和明胶（Gel）为聚阳离子和聚阴离子，通过层层自组装技术在聚L-乳酸（PLLA）静电纺丝纤维上构建了多层膜。首先通过聚（乙烯亚胺）（PEI）在PLLA骨架中的酯的氨解反应产生带正电荷的表面，然后随后交替沉积凝胶和CS。FTIR和XPS测量证实了凝胶和CS的替代涂层，而SEM观察表明支架在PEM开发过程中保持了多孔和纤维状形态。IMARIS软件，这是广泛用于重建三维图像，被用来可视化的神经元网络的架构和单个神经元的神经突起的发展。我们的研究结果表明，质子交换膜修饰显着增强细胞-基质的相互作用，提高细胞活力和神经突起的生长。与PLLA纤维支架和经Gel或CS单层修饰的支架相比，Gel/CS PEM涂层纤维支架上获得的神经突起分支明显增多，突起长度明显延长。此外，最外层的组分显示出对神经元生长的影响，在更高的细胞活力以及更多的分支和更长的神经突上获得的凝胶最外层涂覆的纤维支架上比那些最外层涂覆有CS。本研究表明，多糖-蛋白质组装多层膜可用于聚酯纤维的表面改性，为神经组织工程仿生支架的制备提供了一种新的思路。
In this study, we present a simple yet efficient method to modify polyester electrospun fibrous scaffolds by polysaccharide-protein multilayer formation based on electrostatic interactions. Chitosan (CS) and gelatin (Gel) was selected as the polycation and polyanion, respectively, to build polyelectrolyte multilayers (PEMs) on poly-L-lactide (PLLA) electrospun fibers via layer-by-layer (LbL) self-assembly technique. A positively charged surface was first created via the aminolysis reaction of esters in the PLLA backbone by poly(ethylene imine) (PEI), followed by consequent alternate deposition of Gel and CS. FTIR and XPS measurements confirmed alternative coating of Gel and CS, while SEM observation indicated that the scaffolds maintained the porous and fibrous morphology during PEM development. IMARIS software, which was extensively employed to reconstruct 3D images, was employed to visualize the architecture of neuronal networks and neurite development of single neurons. Our results indicated that the PEM-modification significantly enhanced cell-matrix interactions by improving cell viability and neurite outgrowth. Significantly more branches and longer neurites were obtained on Gel/CS PEM-coated fibrous scaffolds than PLLA fibrous scaffold and those after Gel or CS monolayer modification. Moreover, the component of the outermost layer showed influence on neuron growth in terms of higher cell viability as well as more branches and longer neurites being obtained on Gel-outermost coated fibrous scaffolds than those outermost coated with CS. The current study indicated that the polysaccharide-protein assembly multilayer could be employed to modify the surface of polyester fibers, thus providing a new strategy to fabricate biomimetic scaffolds for nerve tissue engineering.