Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors.
Adhesion, migration, and homing of murine hematopoietic stem cells and progenitors.
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DOI：
10.1007/978-1-61779-145-1_13
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发表时间：
2011
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影响因子：
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通讯作者：
Cancelas, Jose A
中科院分区：
文献类型：
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作者：
Cancelas, Jose A
细胞生物学的最新进展已经证明了多种信号传导蛋白在将外部信号转导至细胞质和细胞核效应物中的作用，控制造血干细胞和祖细胞（HSC/P）在骨髓内的运动和/或保留，具有重要的临床意义。在分析与微环境的粘附、向化学引诱物梯度的迁移以及体内骨髓中HSC/P的归巢方面，多种测定已成为常规方法。在这一章中，我们分析了一些最常用的检测在我们的实验室，探讨HSC迁移的能力，坚持，并在体外和体内测定家。
Recent advances in cell biology have demonstrated the role of multiple signaling proteins in the transduction of external signals to cytoplasmic and nuclear effectors, controlling the movement and/or retention of hematopoietic stem cells and progenitors (HSC/P) within the bone marrow, with important clinical implications. Multiple assays have become routine in the analysis of adhesion to the microenvironment, migration toward chemoattractant gradients, and homing of HSC/P in the bone marrow in vivo. In this chapter, we analyze some of the most frequently used assays in our laboratory to explore the ability of HSC to migrate, adhere, and home in in vitro and in vivo assays.