ABC Transporters in Prorocentrum lima and Their Expression Under Different Environmental Conditions Including Okadaic Acid Production
ABC Transporters in Prorocentrum lima and Their Expression Under Different Environmental Conditions Including Okadaic Acid Production
复制标题
利马原甲藻中的 ABC 转运蛋白及其在不同环境条件下的表达（包括冈田酸生产）
DOI：
10.3390/md17050259
复制
发表时间：
2019-05-01
期刊：
影响因子：
5.4
通讯作者：
Yang, Wei-Dong
中科院分区：
文献类型：
--
作者：
Gu, Song;Xiao, Shao-Wen;Yang, Wei-Dong
利马原甲藻（Prorocentrum lima）是一种典型的底栖有毒甲藻，能产生冈田酸（Okadaic acid，OA）等藻毒素。在这项研究中，我们确定了三个ABC转运蛋白基因（ABCB 1，ABCC 1和ABCG 2），并表征了它们的表达模式，以及OA生产在不同的环境条件下，在利马。我们发现这三个ABC转运蛋白与其他物种的相关ABC蛋白具有高度的同源性，并具有ABC转运蛋白的经典特征。其中，ABCG 2是一个半大小的转运体。三个ABC转运蛋白基因在不同条件下表现出不同的表达谱。高浓度Cu 2+可上调利玛原甲藻ABCB 1、ABCC 1和ABCG 2的转录水平，提示ABC转运蛋白可能对周围沃茨中的金属离子具有防御作用。一定浓度的Cu ~（2+）能诱导OA的产生，而三丁基锡则抑制OA的积累。卤虫能够诱导OA的产生，而利马虫对卤虫具有一定的毒性，表明OA具有抗放牧作用。总的来说，我们的研究结果揭示了有趣的数据OA生产和三个ABC转运蛋白基因的表达模式。然而，我们没有发现任何显着的相关性OA生产和表达模式的三个ABC转运蛋白在利马。我们的研究结果可能会提供新的分子见解的防御反应的P. lima周围的环境。
Prorocentrum lima is a typical benthic toxic dinoflagellate, which can produce phycotoxins such as okadaic acid (OA). In this study, we identified three ABC transporter genes (ABCB1, ABCC1 and ABCG2) and characterized their expression patterns, as well as OA production under different environmental conditions in P. lima. We found that the three ABC transporters all showed high identity with related ABC proteins from other species, and contained classical features of ABC transport proteins. Among them, ABCG2 was a half size transporter. The three ABC transporter genes displayed various expression profiles under different conditions. The high concentration of Cu2+ could up-regulate ABCB1, ABCC1 and ABCG2 transcripts in P. lima, suggesting the potential defensive role of ABC transporters against metal ions in surrounding waters. Cu2+, in some concentration, could induce OA production; meanwhile, tributyltin inhibited OA accumulation. The grazer Artemia salina could induce OA production, and P. lima displayed some toxicity to the grazer, indicating the possibility of OA as an anti-grazing chemical. Collectively, our results revealed intriguing data about OA production and the expression patterns of three ABC transporter genes. However, we could not find any significant correlation between OA production and expression pattern of the three ABC transporters in P. lima. Our results might provide new molecular insights on the defensive responses of P. lima to the surrounding environment.