Toward a physical map of human chromosome 10: isolation of 183 YACs representing 80 loci and regional assignment of 94 YACs by fluorescence in situ hybridization.
Toward a physical map of human chromosome 10: isolation of 183 YACs representing 80 loci and regional assignment of 94 YACs by fluorescence in situ hybridization.
复制标题
绘制人类 10 号染色体的物理图谱：通过荧光原位杂交分离代表 80 个基因座的 183 个 YAC，并对 94 个 YAC 进行区域分配。
作者：
Moir,DT;Dorman,TE;Day,JC;Ma,NS;Wang,MT;Mao,JI
通过基于 PCR 的筛选，从多基因组等效文库中分离出 183 个携带人类 10 号染色体序列的 YAC，以确定是否存在 80 种不同的 10 号染色体特异性 STS。 94 个分离的 YAC，代表 52 个基因和 DNA 片段，通过荧光原位杂交定位到 10 号染色体的区域。结果首次将 26 个 DNA 片段定位到细胞遗传学带上。约 37% (35/94) 的 YAC 与多个染色体位置杂交：除了 10 号染色体外，还有 31 条与其他染色体杂交，以及 10 号染色体上的 4 至 2 个不同位置杂交。这些结果与这些文库中预期的嵌合 YAC 数量一致，但也可能反映出单个克隆内存在 2 个或更多 YAC，或者基因组内存在低拷贝重复元件。该 STS 锚筛选工作鉴定出了 69 个重叠群，其中 7 个重叠群各由 2 个锚定组成，1 个重叠群由 5 个锚定组成。所有链接的 STS 均由至少 2 个独立的 YAC 多重链接。这些锚定的 YAC 跨越整个染色体，似乎覆盖了 10 号染色体的 15%。
One hundred eighty-three YACs carrying human chromosome 10 sequences were isolated from multigenome equivalent libraries by PCR-based screening for the presence of 80 different chromosome 10-specific STSs. Ninety-four of the isolated YACs, representing 52 genes and DNA segments, were mapped to regions of chromosome 10 by fluorescencein situhybridization. The results localized 26 DNA segments to cytogenetic bands for the first time. About 37% (35/94) of the YACs hybridized to more than one chromosomal location: 31 to other chromosomes in addition to chromosome 10 and 4 to 2 distinct locations on chromosome 10. These results are consistent with the number of chimeric YACs expected from these libraries but may also reflect the presence of 2 or more YACs within a single clone or the presence of low copy repeated elements within the genome. This STS anchor screening effort resulted in the identification of 69 contigs, with 7 contigs consisting of 2 anchors each and 1 contig consisting of 5 anchors. All linked STSs were multiply linked by at least 2 independent YACs. These anchored YACs span the entire chromosome and appear to cover 15% of chromosome 10.