Table grape consumption reduces adiposity and markers of hepatic lipogenesis and alters gut microbiota in butter fat-fed mice.
Table grape consumption reduces adiposity and markers of hepatic lipogenesis and alters gut microbiota in butter fat-fed mice.
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DOI：
10.1016/j.jnutbio.2015.08.027
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发表时间：
2016-01
期刊：
影响因子：
--
通讯作者：
McIntosh MK
中科院分区：
文献类型：
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作者：
Baldwin J;Collins B;Wolf PG;Martinez K;Shen W;Chuang CC;Zhong W;Cooney P;Cockrell C;Chang E;Gaskins HR;McIntosh MK
我们的目标是确定食用鲜食葡萄是否会减少肥胖及其代谢后果，并改变喂食高脂肪（HF），富含黄油饮食的小鼠的肠道微生物群。C57 BL/6 J小鼠喂食低脂肪（LF）饮食或含3%或5%葡萄的HF饮食11周。总身体和腹股沟脂肪是适度的，但显着减少小鼠喂养两个水平的葡萄相比，他们的控制。与5%的对照组相比，喂食5%葡萄的小鼠肝脏重量和甘油三酯水平较低，甘油-3-磷酸酰基转移酶（Gpat 1）的表达也较低。与3%的对照组相比，喂食3%葡萄的小鼠肝脏过氧化物酶体增殖物激活受体γ 2、甾醇-CoA去饱和酶1、脂肪酸结合蛋白4和Gpat 1的mRNA水平较低。虽然葡萄喂养对脂肪组织或肠道中炎症或脂肪生成的标志物只有很小的影响，但3%的葡萄降低了肠道中产硫脱硫菌的丰度，和嗜盐菌特异性异化亚硫酸盐还原酶基因（dsrA-Bw），并且与对照相比倾向于增加有益细菌嗜粘蛋白阿克曼氏菌的丰度。此外，双歧杆菌，乳酸杆菌，Allobaculum，和其他几个属与肥胖呈负相关。与HF饲喂的对照组相比，LF和3%葡萄组中的Allobaculum特别增加。值得注意的是，葡萄喂养衰减HF诱导的损伤上皮定位的肠紧密连接蛋白封闭小带。总的来说，这些数据表明，食用富含饱和脂肪的HF饮食的一些不良健康后果可以通过食用葡萄来减轻。
Our objective was to determine if consuming table grapes reduces adiposity and its metabolic consequences and alters gut microbiota in mice fed a high fat (HF), butter-rich diet. C57BL/6J mice were fed a low fat (LF) diet or HF diet with 3% or 5% grapes for 11 weeks. Total body and inguinal fat were moderately, but significantly reduced in mice fed both levels of grapes compared to their controls. Mice fed 5% grapes had lower liver weights and triglyceride levels, and decreased expression of glycerol-3-phosphate acyltransferase (Gpat1) compared to the 5% controls. Mice fed 3% grapes had lower hepatic mRNA levels of peroxisome proliferator-activated receptor gamma 2, sterol-CoA desaturase 1, fatty-acid binding protein 4, and Gpat1 compared to the 3% controls. Although grape feeding had only a minor impact on markers of inflammation or lipogenesis in adipose tissue or intestine, 3% grapes decreased the intestinal abundance of sulfidogenic Desulfobacter spp., and the Bilophila wadsworthia-specific dissimilatory sulfite reductase gene (dsrA-Bw), and tended to increase the abundance of the beneficial bacterium Akkermansia muciniphila compared to controls. Additionally, Bifidobacterium, Lactobacillus, Allobaculum, and several other genera correlated negatively with adiposity. Allobaculum in particular was increased in the LF and 3% grapes groups compared to the HF-fed controls. Notably, grape feeding attenuated the HF-induced impairment in epithelial localization of the intestinal tight junction protein zonula occludens. Collectively, these data indicate that some of the adverse health consequences of consuming a HF diet rich in saturated fat can be attenuated by table grape consumption.