The Role of Basic Fibroblast Growth Factor in Skeletal Muscle Regeneration
The Role of Basic Fibroblast Growth Factor in Skeletal Muscle Regeneration
复制标题
DOI：
10.3109/08977199209008871
复制
发表时间：
1992-01-01
期刊：
影响因子：
1.8
通讯作者：
Hearn, Milton T. W.
中科院分区：
文献类型：
--
作者：
Guthridge, Mark;Wilson, Michael;Hearn, Milton T. W.
肌肉的生长和再生是由局部产生的生长因子控制的，这些生长因子激活卫星细胞并刺激其增殖、分化和融合形成成熟的肌管。碱性成纤维细胞生长因子（bFGF）在体外已被证明能促进成肌细胞的增殖并抑制其分化。相比之下，这种生长因子在体内的作用还没有得到很好的证明。在目前关于bFGF在肌肉再生中的作用的研究中，我们在两个实验系统中研究了bFGF mRNA的水平：(1)来源于大鼠骨骼肌的原代细胞培养，(2)体内大鼠肌肉损伤模型。在融合前的成肌细胞和原代肌细胞培养的肌管中检测到bFGF mRNA。在未损伤的肌肉中，肌管中检测到bFGF mRNA转录物，而卫星细胞中未检测到。在体内肌肉损伤模型中，在成肌细胞、变性肌管和再生肌管中观察到bFGF mRNA的表达。讨论了这些实验结果对bFGF在体内肌生成程序中所起作用的意义。
Muscle growth and regeneration is controlled by locally produced growth factors which activate satellite cells and stimulate their proliferation, differentiation and fusion to form mature myotubes. Basic fibroblast growth factor (bFGF) has been previously shown to promote proliferation and inhibit differentiation of myoblasts in vitro. In comparison, the in vivo role of this growth factor is less well documented. In the present investigation on the role of bFGF in muscle regeneration, bFGF mRNA levels were studied in two experimental systems: (1) primary cell cultures derived from rat skeletal muscles, and (2) an in vivo rat muscle injury model. bFGF mRNA was detected in myoblasts just prior to fusion and in myotubes of primary muscle cell cultures. In the non-injured muscle, bFGF mRNA transcripts were detected in myotubes but not satellite cells. In the in vivo muscle injury model bFGF mRNA was observed in myoblasts and in degenerating and regenerated myotubes. The significance of these experimental results in terms of the role played by bFGF in the myogenic program in vivo are discussed.