Japanese subgingival microbiota in health vs disease and their roles in predicted functions associated with periodontitis
Japanese subgingival microbiota in health vs disease and their roles in predicted functions associated with periodontitis
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DOI：
10.1007/s10266-019-00452-4
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发表时间：
2020-04-01
期刊：
影响因子：
2.5
通讯作者：
Izumi, Yuichi
中科院分区：
文献类型：
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作者：
Ikeda, Eri;Shiba, Takahiko;Izumi, Yuichi
本研究旨在利用16S rRNA基因的454个序列，鉴定和比较牙周健康者和牙周炎者的微生物特征。从10名牙周健康受试者和10名匹配的慢性牙周炎患者中收集龈下菌斑样本。提取细菌DNA，对16S rRNA基因进行下一代测序。在所有系统发育水平上，健康受试者与牙周炎患者的微生物组成存在差异。特别是，在健康受试者中，包括laurtropia mirabilis和Neisseria subflava在内的16种占优势，而在牙周炎受试者中，包括牙龈卟啉单胞菌和alocfilifactor alocis在内的9种占优势。UniFrac是一项主要的坐标和网络分析，证实了健康受试者和牙周炎患者的不同社区概况。通过预测功能分析，在牙周炎患者中，涉及苯丙氨酸、gpi锚定生物合成和丙氨酸、精氨酸、天冬氨酸、丁酸盐、氰氨基酸、脂肪酸、谷氨酸、甲烷、脯氨酸和维生素B6代谢的途径明显过多。这些结果强调了牙周炎患者口腔微生物组成的改变，并提示了与健康和牙周炎相关的基因和代谢途径。我们的发现有助于进一步阐明健康和牙周炎中的微生物组成和相互作用。
The present study aimed to identify and compare the microbial signatures between periodontally healthy and periodontitis subjects using 454 sequences of 16S rRNA genes. Subgingival plaque samples were collected from ten periodontally healthy subjects and ten matched chronic periodontitis patients. Bacterial DNA was extracted and next-generation sequencing of 16S rRNA genes was performed. The microbial composition differed between healthy subjects and periodontitis patients at all phylogenetic levels. Particularly, 16 species, including Lautropia mirabilis and Neisseria subflava predominated in healthy subjects, whereas nine species, including Porphyromonas gingivalis and Filifactor alocis predominated in periodontitis. UniFrac, a principal coordinate and network analysis, confirmed distinct community profiles in healthy subjects and periodontitis patients. Using predicted function profiling, pathways involved in phenylpropanoid, GPI-anchor biosynthesis, and metabolism of alanine, arginine, aspartate, butanoate, cyanoamino acid, fatty acid, glutamate, methane, proline, and vitamin B6 were significantly over-represented in periodontitis patients. These results highlight the oral microbiota alterations in microbial composition in periodontitis and suggest the genes and metabolic pathways associated with health and periodontitis. Our findings help to further elucidate microbial composition and interactions in health and periodontitis.