Substrate range of the 40,000-dalton DNA-photoreactivating enzyme from Escherichia coli.
Substrate range of the 40,000-dalton DNA-photoreactivating enzyme from Escherichia coli.
复制标题
来自大肠杆菌的 40,000 道尔顿 DNA 光复活酶的底物范围。
DOI：
10.1021/bi00351a026
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发表时间：
1986
期刊：
影响因子：
2.9
通讯作者：
Hanawalt,PC
中科院分区：
文献类型：
--
作者：
Sutherland,BM;Oliveira,OM;Ciarrocchi,G;Brash,DE;Haseltine,WA;Lewis,RJ;Hanawalt,PC
Roger J. lewis和Philip C. Hanawalt，斯坦福大学生物科学系，Stanford University, California 94305, June 13, 1985摘要：我们测定了40,000道尔顿大肠杆菌光再激活酶对核酸中多种嘧啶-嘧啶光产物底物的作用能力。该酶对超螺旋DNA中的间合-环丁基嘧啶二聚体的活性至少与在线性DNA中的活性一样，但对RNA中的二聚体无活性。二聚体嘧啶脱氧核糖内部的磷酸二酯键和连接嘧啶和脱氧核糖的v -糖基键都必须是完整的，才能使酶起作用。该酶对DNA中的（6-4）嘧啶-胞嘧啶产物没有活性。^光解酶（ec4.1）99.3)修复紫外线（UV, 220-310 nm）照射DNA，在光（300-600 nm）依赖反应中单体化c/s ' - ijin -环丁基嘧啶二聚体（PD） （Setlow， J. K., et, 1965; Setlow， R. B., et, 1965）。这些酶的明显特异性（Setlow & Setlow, 1963）使他们的
Roger J. Lewis1 and Philip C. Hanawalt Department of Biological Sciences, Stanford University, Stanford, California 94305 Received June 13, 1985 abstract: We determined the ability of the 40000-dalton Escherichia coli photoreactivating enzyme to act on a variety of pyrimidine-pyrimidinephotoproduct substrates in nucleic acids. The enzyme is at least as active on m-syn-cyclobutylpyrimidine dimers in supercoiled DNA as in linear DNA, but inactive on dimers in RNA. Both the phosphodiester bond internal to the deoxyriboses of the pyrimidines of the dimer and the-V-glycosyl bond joining the pyrimidine to deoxyribose must be intact for enzyme action. The enzyme has no activity toward (6-4) pyrimidine-cytosine products in DNA.I^ iotoreactivating enzymes (photolyase, EC 4.1. 99.3) repair ultraviolet light (UV, 220-310 nm) 1 irradiated DNA by monomerizing c/s’-ijn-cyclobutylpyrimidine dimers (PD) in a light (300-600 nm)-dependent reaction (Setlow, J. K., et al., 1965; Setlow, R. B., et al., 1965). The apparent specificity of these enzymes (Setlow & Setlow, 1963) has allowed their