DE-NOVO FORMATION OF CAVEOLAE IN LYMPHOCYTES BY EXPRESSION OF VIP21-CAVEOLIN
DE-NOVO FORMATION OF CAVEOLAE IN LYMPHOCYTES BY EXPRESSION OF VIP21-CAVEOLIN
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DOI：
10.1073/pnas.92.19.8655
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发表时间：
1995-09-12
影响因子：
11.1
通讯作者：
PARTON, RG
中科院分区：
文献类型：
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作者：
FRA, AM;WILLIAMSON, E;PARTON, RG
小窝是细胞膜内陷，与内皮细胞转胞吞、内吞、光胞吞和信号转导有关。除了它们明确定义的形态外，小窝的特征在于存在称为VIP 21-小窝蛋白的整合膜蛋白。我们最近观察到，淋巴细胞没有可检测到的VIP 21-小窝蛋白和缺乏质膜内陷类似小窝。在这里，我们瞬时表达VIP 21-小窝蛋白的淋巴细胞系使用Semliki森林病毒表达系统，并显示从头形成的质膜内陷含有VIP 21-小窝蛋白。这些内陷出现均匀的大小和形态学上无法区分的小窝的nonlymphoid细胞，此外，糖基磷脂酰肌醇锚定蛋白Thy 1，修补的抗体，重新分配到新形成的小窝，我们的研究结果表明，VIP 21-小窝蛋白是一个关键的结构组成部分所需的小窝的生物发生。
Caveolae are plasma membrane invaginations, which have been implicated in endothelial transcytosis, endocytosis, potocytosis, and signal transduction. In addition to their well-defined morphology, caveolae are characterized by the presence of an integral membrane protein termed VIP21-caveolin. We have recently observed that lymphocytes have no detectable VIP21-caveolin and lack plasma membrane invaginations resembling caveolae. Here we transiently express VIP21-caveolin in a lymphocyte cell line using the Semliki Forest virus expression system and show de novo formation of plasma membrane invaginations containing VIP21-caveolin. These invaginations appear homogeneous in size and morphologically indistinguishable from caveolae of nonlymphoid cells, Moreover, the glycosylphosphatidylinositol-anchored protein Thy1, patched by antibodies, redistributes to the newly formed caveolae, Our results show that VIP21-caveolin is a key structural component required for caveolar biogenesis.