The V delta 1 T cell receptor repertoire in human small intestine and colon.
The V delta 1 T cell receptor repertoire in human small intestine and colon.
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DOI：
10.1084/jem.180.1.183
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发表时间：
1994-07-01
期刊：
影响因子：
--
通讯作者：
Kagnoff MF
中科院分区：
文献类型：
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作者：
Chowers Y;Holtmeier W;Harwood J;Morzycka-Wroblewska E;Kagnoff MF
携带 V δ 1 的 T 细胞构成了人类肠道中主要的 γ/δ T 细胞群。为了深入了解这些细胞的生成机制及其多样性，我们对人类小肠和结肠中 V δ 1 T 细胞受体转录物的连接序列进行了表征。从沿着小肠或结肠长度的限定区域获得的粘膜活检包含高频率的一个或几个在框架V delta 1序列中相同的序列。在小肠或结肠的整个长度中也重复检测到不太丰富的序列。此外，小肠和结肠中的肠道 V δ 1 库似乎是分隔开的，并且与外周血中的 V δ 1 库没有重叠。每个受试者的小肠和结肠之间的显性 V delta 1 转录本不同，并且这些位点内的显性转录本在个体之间也不同。对每隔 1 年获得的小肠转录本的分析表明，V delta 1 库随着时间的推移是稳定的。大多数 V delta 1 转录物（无论是显性的还是稀有的）都分布在成体肠道几米长的区域，并且随着时间的推移保持稳定，这一事实表明它们不是由持续的原位 VDJ 基因重排过程产生的。我们的结果支持这样一种模型，其中肠道中的 V δ 1 T 细胞的库是在 V δ 1 细胞迁移到整个小肠或结肠之前通过响应有限的配体阵列而进行正选择而形成的。
V delta 1 bearing T cells comprise the major population of gamma/delta T cells in the human intestinal tract. To gain insight into mechanisms involved in the generation of these cells and the diversity of their repertoire, we have characterized the junctional sequences of V delta 1 T cell receptor transcripts in the human small intestine and colon. Mucosal biopsies obtained from defined regions along the length of the small intestine or colon contained a high frequency of either one or a few identical in frame V delta 1 sequences. Less abundant sequences were also detected repeatedly throughout the length of small intestine or colon. Moreover, the intestinal V delta 1 repertoire in the small intestine and colon appeared compartmentalized and showed no overlap with the V delta 1 repertoire in peripheral blood. Dominant V delta 1 transcripts in each subject differed between the small intestine and colon, and the dominant transcripts within these sites differed among individuals. Analysis of small intestinal transcripts obtained at a 1- yr interval revealed that the V delta 1 repertoire was stable over time. The fact that the majority of V delta 1 transcripts, both dominant and rare, are distributed throughout a several meter length of the adult intestinal tract and are stable over time suggests they are not generated by an ongoing process of in situ VDJ gene rearrangement. Our results favor a model in which the repertoire of V delta 1 T cells in the intestinal tract is shaped by positive selection in response to a limited array of ligands before the migration of V delta 1 cells throughout the small intestine or colon.