Extracellular matrix proteins of dentine.
Extracellular matrix proteins of dentine.
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牙本质的细胞外基质蛋白。
DOI：
10.1002/9780470515303.ch8
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
A. Bronckers
中科院分区：
文献类型：
--
作者：
William T. Butler;Helena H. Ritchie;A. Bronckers
骨和牙本质细胞外基质蛋白是相似的，主要由I型胶原、酸性蛋白和蛋白聚糖组成。虽然胶原形成钙和磷酸盐沉积的晶格以形成碳酸磷灰石，但据信非胶原蛋白控制晶体的起始和生长。尽管有这种相似性，牙本质含有三种独特的蛋白质，显然不存在于骨和其他组织中：牙本质磷酸蛋白（DPP），牙本质基质蛋白1（DMP 1）和牙本质唾液蛋白（DSP）。DPP和DMP 1是酸性磷蛋白，可能参与矿化过程的控制。DPP可能定位于胶原蛋白的间隙区域，并通过以促进该过程的构象结合大量钙来启动磷灰石晶体形成。本实验室对DSP的性质、生物合成、定位和基因结构进行了广泛的研究。免疫定位研究表明，大鼠DSP，一个53 kDa的唾液酸丰富的糖蛋白，合成的年轻和成熟的成牙本质细胞，牙髓细胞和前成釉细胞，但不是由成釉细胞，成骨细胞，软骨细胞或其他类型的细胞。cDNA序列表明DSP是一个366个残基的蛋白质，具有几个潜在的N-糖基化位点，以及磷酸化位点，但氨基酸序列与其他已知蛋白质不同。北方印迹分析在4.6和1.5 kb附近检测到几种mRNA种类，表明选择性剪接事件。获得了两个DSP基因的证据，使这一情况进一步复杂化。最近的原位杂交研究利用大鼠和小鼠磨牙和切牙表明，DSP mRNA表达的年轻成牙本质细胞和成牙本质细胞在所有年龄段的动物。在前成釉细胞中也观察到转录本。当这些细胞成熟成为分泌型成釉细胞时，DSP mRNA的表达停止。在成骨细胞或其他细胞类型中未检测到DSP转录物。在前成釉细胞中的瞬时表达表明上皮-间充质相互作用在牙齿形成中的作用。
Bone and dentine extracellular matrix proteins are similar, consisting primarily of type I collagen, acidic proteins and proteoglycans. Although collagen forms the lattice for deposition of calcium and phosphate for formation of carbonate apatite, the non-collagenous proteins are believed to control initiation and growth of the crystals. Despite this similarity, dentine contains three unique proteins apparently absent from bone and other tissue: dentine phosphophoryn (DPP), dentine matrix protein 1 (DMP1) and dentine sialoprotein (DSP). DPP and DMP1 are acidic phosphoproteins probably involved in the control of mineralization processes. DPP may localize in gap regions of collagen and initiate apatite crystal formation by binding large quantities of calcium in a conformation that promotes this process. Extensive studies have been conducted in our laboratory on the nature, biosynthesis, localization and gene structure of DSP. Immunolocalization studies showed that rat DSP, a 53 kDa sialic acid-rich glycoprotein, was synthesized by young and mature odontoblasts, and by dental pulp cells and pre-ameloblasts, but not by ameloblasts, osteoblasts, chondrocytes or other cell types. The cDNA sequence indicated that DSP was a 366-residue protein with several potential N-glycosylation sites, as well as phosphorylation sites, but that the amino acid sequence was dissimilar to that of other known proteins. Northern blot analysis detected several mRNA species near 4.6 and 1.5 kb, indicative of alternative splicing events. Evidence for two DSP genes was obtained, further complicating this picture. Recent in situ hybridization studies utilizing rat and mouse molars and incisors indicated that DSP mRNA was expressed by young odontoblasts and odontoblasts in animals of all ages. Transcripts were also observed in pre-ameloblasts. The expression of DSP mRNA ceased when these cells matured to become secretory ameloblasts. DSP transcripts were not detected in osteoblasts or other cell types. The transient expression in pre-ameloblasts suggests a role of epithelial-mesenchymal interactions in the formation of the tooth.
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DOI：
10.1016/s0021-9258(18)31434-0
发表时间：
1993-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Anne George;B. Sabsay;Philip A L Simonian;A. Veis
通讯作者：
Anne George;B. Sabsay;Philip A L Simonian;A. Veis
钙离子和磷酸根离子与牙本质磷蛋白的结合。
影响因子：
2.9
作者：
Marsh,ME
通讯作者：
Marsh,ME
DOI：
10.1016/s0934-8832(11)80030-2
发表时间：
1992-11-01
期刊：
MATRIX
影响因子：
--
作者：
BUTLER, WT;BHOWN, M;VANDIJK, S
通讯作者：
VANDIJK, S
影响因子：
3
作者：
LANDIS, WJ;SONG, MJ;MCEWEN, BF
通讯作者：
MCEWEN, BF
DOI：
10.1387/ijdb.7626404
发表时间：
1995-02
期刊：
The International journal of developmental biology
影响因子：
--
作者：
W. Butler;H. Ritchie
通讯作者：
W. Butler;H. Ritchie