Developmental mucin gene expression in the gastroduodenal tract and accessory digestive glands. II. Duodenum and liver, gallbladder, and pancreas
Developmental mucin gene expression in the gastroduodenal tract and accessory digestive glands. II. Duodenum and liver, gallbladder, and pancreas
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DOI：
10.1177/002215540004801210
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发表时间：
2000-12-01
影响因子：
3.2
通讯作者：
Porchet, N
中科院分区：
文献类型：
--
作者：
Buisine, MP;Devisme, L;Porchet, N
研究提供了关于粘蛋白基因的细胞特异性表达及其与上皮细胞分化的发育和肿瘤模式的关系的信息。用原位杂交法研究了13例人胚胎和胎儿(6.5~27周)十二指肠和副消化腺(肝、胆、胰腺)中粘蛋白基因的表达，并与正常组织和肿瘤成人组织进行了比较。这些研究表明，胎儿十二指肠粘蛋白基因的表达模式与我们在胃和肠道个体发育过程中观察到的模式相同，MUC2和MUC3在表面上皮细胞中表达，MUC6表达与Brunner腺体的发育有关。在胚胎肝脏中，MUC3在妊娠6.5周时已在肝母细胞中表达。与成人一样，MUC1、MUC2、MUC3、MUC5AC、MUC5B和MUC6在胎儿胆囊中表达，而MUC4不表达。相反，MUC4在胆囊腺癌中呈强阳性表达。MUC5B和MUC6分别在妊娠12周和26周的胎儿胰腺中表达。令人惊讶的是，在成人胰腺中强表达的MUC3在发育中的胰腺中未检测到。综上所述，这些数据显示了粘蛋白基因复杂的时空调控，并提示粘蛋白基因产物在胃十二指肠上皮细胞分化中可能发挥调节作用。
Studies were undertaken to provide information regarding cell-specific expression of mucin genes and their relation to developmental and neoplastic patterns of epithelial cytodifferentiation. In situ hybridization was used to study mRNA expression of mucin genes in duodenum and accessory digestive glands (liver, gallbladder, pancreas) of 13 human embryos and fetuses (6.5-27 weeks' gestation), comparing these with normal and neoplastic adult tissues. These investigations demonstrated that the pattern of mucin gene expression in fetal duodenum reiterated the patterns we observed during gastric and intestinal ontogenesis, with MUC2 and MUC3 expression in the surface epithelium and MUC6 expression associated with the development of Brunner's glands. In embryonic liver, MUC3 was already expressed at 6.5 weeks of gestation in hepatoblasts. As in adults, MUC1, MUC2, MUC3, MUC5AC, MUC5B, and MUC6 were expressed in fetal gallbladder, whereas MUC4 was not. In contrast, MUC4 was strongly expressed in gallbladder adenocarcinomas. MUC5B and MUC6 were expressed in fetal pancreas, from 12 weeks and 26 weeks of gestation, respectively. Surprisingly, MUC3 which is strongly expressed in adult pancreas, was not detected in developmental pancreas. Taken together, these data show complex spatiotemporal regulation of the mucin genes and suggest a possible regulatory role for mucin gene products in gastroduodenal epithelial cell differentiation.