A comparison of the effects of male pheromone priming and optogenetic inhibition of accessory olfactory bulb forebrain inputs on the sexual behavior of estrous female mice.
A comparison of the effects of male pheromone priming and optogenetic inhibition of accessory olfactory bulb forebrain inputs on the sexual behavior of estrous female mice.
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DOI：
10.1016/j.yhbeh.2016.12.011
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发表时间：
2017-03
影响因子：
3.5
通讯作者：
Baum MJ
中科院分区：
文献类型：
--
作者：
McCarthy EA;Kunkhyen T;Korzan WJ;Naik A;Maqsudlu A;Cherry JA;Baum MJ
先前的研究表明，卵巢切除、激素引发的雌性小鼠需要用雄性刺激物进行重复测试才能变得具有性接受能力（显示最大前凸商；LQ），并且药物诱导的雌二醇受体功能的表观遗传增强加速了LQ的改善，否则发情雌性通过重复测试会显示出LQ的改善。我们通过重复测试询问预先暴露于雄性信息素（“信息素启动”）是否也会加速 LQ 的改善，以及辅助嗅球（AOB）投射神经元的光遗传学抑制是否可以抑制有性经历的发情雌性小鼠的脊柱前凸。在实验 1 中，用肮脏的雄性垫料重复启动并不能加速发情雌性小鼠在 5 次测试中显示的 LQ 的逐步改善，尽管这种启动增加了第一次测试期间每次脊柱前凸反应的持续时间和雌性对雄性身体部位的调查。在实验2中，在自由活动的行为测试期间，对前脑AOB输入的急性光遗传学抑制显着降低了LQ，这表明即使在有性经验的雌性中，交配期间持续向前脑发送AOB信号也是最大脊柱前凸反应所必需的。我们的研究结果还表明，信息素刺激本身并不能替代发情雌性从雄性雄性那里接收到的全部感觉刺激，而这些刺激通常会通过重复测试导致她们的 LQ 逐渐改善。
Previous research has shown that repeated testing with a stimulus male is required for ovariectomized, hormone-primed female mice to become sexually receptive (show maximal lordosis quotients; LQs) and that drug-induced, epigenetic enhancement of estradiol receptor function accelerated the improvement in LQs otherwise shown by estrous females with repeated testing. We asked whether pre-exposure to male pheromones (‘pheromone priming’) would also accelerate the improvement in LQs with repeated tests and whether optogenetic inhibition of accessory olfactory bulb (AOB) projection neurons could inhibit lordosis in sexually experienced estrous female mice. In Experiment 1, repeated priming with soiled male bedding failed to accelerate the progressive improvement in LQs shown by estrous female mice across 5 tests, although the duration of each lordosis response and females’ investigation of male body parts during the first test was augmented by such priming. In Experiment 2, acute optogenetic inhibition of AOB inputs to the forebrain during freely moving behavioral tests significantly reduced LQs, suggesting that continued AOB signaling to the forebrain during mating is required for maximal lordotic responsiveness even in sexually experienced females. Our results also suggest that pheromonal stimulation, by itself, cannot substitute for the full complement of sensory stimulation received by estrous females from mounting males that normally leads to the progressive improvement in their LQs with repeated testing.