Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from Klebsiella pneumoniae and comparison with a related Streptococcus pneumoniae complex.
Structure of a quinolone-stabilized cleavage complex of topoisomerase IV from Klebsiella pneumoniae and comparison with a related Streptococcus pneumoniae complex.
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DOI：
10.1107/s2059798316001212
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Sanderson MR
中科院分区：
文献类型：
--
作者：
Veselkov DA;Laponogov I;Pan XS;Selvarajah J;Skamrova GB;Branstrom A;Narasimhan J;Prasad JV;Fisher LM;Sanderson MR
拓扑异构酶Ⅳ裂解复合物的晶体结构。肺炎链球菌）和革兰氏阳性（S.肺炎）细菌病原体稳定的临床重要的抗菌药物左氧氟沙星，分析和比较。克雷肺炎，这是第一个高分辨率的切割复合物结构被报道。 肺炎克雷伯氏菌是一种革兰氏阴性细菌，可引起一系列常见感染，包括肺炎、血流感染和脑膜炎。克雷伯氏菌的某些菌株对抗生素具有高度耐药性。尽管对这类细菌进行了大量研究，但其拓扑异构酶IV（催化染色体分离所必需的第二型拓扑异构酶）的分子结构仍然未知。本文报道了它的DNA切割复合物的结构，分辨率为3.35 μ m。 该复合物由K的ParC断裂-重聚结构域和帕雷TOPRIM结构域组成。肺炎拓扑异构酶IV与左氧氟沙星（一种广谱氟喹诺酮类抗微生物剂）稳定的DNA。这个复杂的比较与一个类似的复杂的肺炎链球菌，最近已经解决。
Crystal structures of the cleavage complexes of topoisomerase IV from Gram-negative (K. pneumoniae) and Gram-positive (S. pneumoniae) bacterial pathogens stabilized by the clinically important antibacterial drug levofloxacin are presented, analysed and compared. For K. pneumoniae, this is the first high-resolution cleavage complex structure to be reported. Klebsiella pneumoniae is a Gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. Certain strains of Klebsiella have become highly resistant to antibiotics. Despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase IV, a type II topoisomerase essential for catalysing chromosomal segregation, had remained unknown. In this paper, the structure of its DNA-cleavage complex is reported at 3.35 Å resolution. The complex is comprised of ParC breakage-reunion and ParE TOPRIM domains of K. pneumoniae topoisomerase IV with DNA stabilized by levofloxacin, a broad-spectrum fluoroquinolone antimicrobial agent. This complex is compared with a similar complex from Streptococcus pneumoniae, which has recently been solved.
影响因子：
3.7
作者：
Sohi MK;Veselkov DA;Laponogov I;Pan XS;Fisher LM;Sanderson MR
通讯作者：
Sanderson MR